早期人胎盘泌乳素分泌的体外调控。

A Szilágyi, A K Mukhopadhyay, J Werling, I Szabó
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引用次数: 0

摘要

肾素-血管紧张素系统的所有组成部分在人胎盘内的存在似乎是很好的确定,但它们的功能和调节仍然不清楚。为了探讨这一问题,采用Medium 199培养基培养早期人胎盘(妊娠9-12周)胎盘组织外植体(50mg)。在每日更换的培养基中,用直接免疫放射法测定Prorenin。在进一步的一系列实验中,以10(-4)-10(-8)mol/l的最终浓度向培养基中添加黄体酮、黄体酮拮抗剂(ZK 98,299)或雌二醇。在早期人胎盘外植体培养基中检测到Prorenin,孕酮使Prorenin值呈剂量依赖性降低。黄体酮拮抗剂和雌二醇也能抑制prorenin的分泌,但抑制作用不明显。黄体酮与黄体酮拮抗剂以等摩尔浓度联合给药时,催乳素分泌减少,但达不到单用黄体酮的抑制作用。由此可见,人胎盘早期存在催乳素活性,催乳素的分泌可能受到孕酮和雌二醇的影响。类固醇激素对催乳素分泌的调节部分类似于人绒毛膜促性腺激素的调节,但其生理意义有待进一步探讨。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Regulation of placental prorenin secretion from the early human placenta in vitro.

The presence of all components of the renin-angiotensin system within the human placenta appears to be well established, but their function and regulation remain obscure. In order to approach this question, placental tissue explants (50 mg) from early human placenta (9-12 weeks of gestation) were cultured in Medium 199. Prorenin was measured by direct immunoradiometric assay in the daily replaced culture medium. In a further series of experiments, progesterone, a progesterone antagonist (ZK 98,299) or estradiol was added to the medium at final concentrations of 10(-4)-10(-8) mol/l. Prorenin was detectable in the culture medium of early human placental explants, and prorenin values were decreased by progesterone in a dose-dependent manner. Progesterone antagonist and estradiol could also inhibit prorenin secretion, but the inhibition was less prominent. When progesterone and progesterone antagonist were co-administered at equimolar concentration, a decrease in prorenin secretion occurred, but it could not reach the inhibitory effect of progesterone given alone. It is concluded that prorenin activity is present in the early human placenta, and prorenin secretion may be influenced by progesterone and estradiol. Regulation of prorenin secretion by steroid hormones is partly similar to that of human chorionic gonadotropin, but its physiological significance awaits further exploration.

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