以多个内部和末端2,4-二硝基苯基标记的寡核苷酸的抗体介导检测和物理性质。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1996-01-01

J McClean, A Davison, M V Rao, T Brown

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引用次数: 0

摘要

DNP标记的磷酰胺已被用于合成具有多个DNP报告基团的寡核苷酸。抗体介导的检测和这些标记的寡核苷酸形成的双链的稳定性进行了研究。dnp标记的脱氧尿苷三磷酸也被用于通过聚合酶链反应将dnp标记酶合到DNA中。在PCR中使用dnp标记引物也进行了研究。

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Antibody-mediated detection and physical properties of oligonucleotides labelled with multiple internal and terminal 2,4-dinitrophenyl groups.

DNP-labelled phosphoramidites have been used to synthesis oligonucleotides with multiple DNP reporter groups. The antibody-mediated detection and the stability of duplexes formed by these labelled oligonucleotides have been studied. A DNP-labelled deoxyuridine triphosphate has also been used to enzymatically incorporate DNP-labels into DNA via the polymerase chain reaction. The use of DNP-labelled primers in the PCR has also been investigated.

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Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity

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