胸苷位点标记2,4-二硝基苯基的寡核苷酸的合成。

Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity Pub Date : 1996-01-01

A Davison, J McClean, M V Rao, T Brown

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引用次数: 0

摘要

在固相合成过程中，制备了一种用DNP基团在胸腺嘧啶位点标记寡核苷酸的磷酸酰胺。二硝基苯基通过一个己胺丙基连接到尿嘧啶的5位上。合成了一种相关的np标记磷酸酰胺，其中丙基被丙基取代。这两种磷酰胺已被用于合成dnp标记的寡核苷酸。一个相关的dnp标记的脱氧尿苷三磷酸也被合成。DNP标记的寡核苷酸探针在基于抗体的DNA和RNA检测的诊断应用中是有价值的。

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Synthesis of oligonucleotides labelled with 2,4-dinitrophenyl groups at thymidine sites.

A phosphoramidite has been produced for labelling oligonucleotides with DNP groups at thymidine sites during solid-phase synthesis. The dinitrophenylamino group is attached via a caproamidopropargyl group to the 5-position of uracil. A related DNP-labelling phosphoramidite has been synthesised where the propargyl group is replaced by propyl. Both phosphoramidites have been used to synthesises DNP-labelled oligonucleotides. A related DNP-labelled deoxyuridine triphosphate has also been synthesised. DNP labelled oligonucleotide probes are valuable in diagnostic applications for the antibody-based detection of DNA and RNA.

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Biomedical peptides, proteins & nucleic acids : structure, synthesis & biological activity

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