前列腺素E2在仓鼠阿米巴肝脓肿形成中的作用

B Sánchez-Ramírez , B Escalante , J.L Rosales-Encina, P Talamás-Rohana
{"title":"前列腺素E2在仓鼠阿米巴肝脓肿形成中的作用","authors":"B Sánchez-Ramírez ,&nbsp;B Escalante ,&nbsp;J.L Rosales-Encina,&nbsp;P Talamás-Rohana","doi":"10.1016/S0090-6980(97)00058-0","DOIUrl":null,"url":null,"abstract":"<div><p>Entamoeba histolytica can modulate macrophage functions and cytokine production by a prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) mechanism. To study the participation of PGE<sub>2</sub> on amoebic liver abscess formation, we tested the effect of the PG synthesis inhibitor indomethacin (INDO) on abscess development in hamsters infected intrahepatically with E. histolytica trophozoites. Male infected animals had higher levels of plasma PGE<sub>2</sub> (5.7 ± 0.7 pg/ml pre-infection; 26.0 ± 2.0 pg/ml 7 days postinfection; p &lt; 0.001). INDO prevented this increase, so that infected-treated and control non-infected animals had similar levels of plasma PGE<sub>2</sub>. INDO reduced liver and abscess weight by 18% and 30% respectively (p &lt; 0.05). Cyclooxygenase (COX) activity determination by thin layer chromatography using (1-<sup>14</sup>C) arachidonic acid (AA) showed that liver microsomes from infected animals produced more PGE<sub>2</sub> than controls. COX activity was considerably inhibited in infected INDO-treated animals. Our data suggest that E. histolytica can stimulate the hepatic production of PGE<sub>2</sub> which contributes to pathogenesis of amoebic abscesses through generation and support of the inflammation. The partial effect of INDO treatment suggests that additional factors are involved.</p></div>","PeriodicalId":20653,"journal":{"name":"Prostaglandins","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1997-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00058-0","citationCount":"27","resultStr":"{\"title\":\"Role of Prostaglandin E2 on Amoebic Liver Abscess Formation in Hamsters\",\"authors\":\"B Sánchez-Ramírez ,&nbsp;B Escalante ,&nbsp;J.L Rosales-Encina,&nbsp;P Talamás-Rohana\",\"doi\":\"10.1016/S0090-6980(97)00058-0\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Entamoeba histolytica can modulate macrophage functions and cytokine production by a prostaglandin E<sub>2</sub> (PGE<sub>2</sub>) mechanism. To study the participation of PGE<sub>2</sub> on amoebic liver abscess formation, we tested the effect of the PG synthesis inhibitor indomethacin (INDO) on abscess development in hamsters infected intrahepatically with E. histolytica trophozoites. Male infected animals had higher levels of plasma PGE<sub>2</sub> (5.7 ± 0.7 pg/ml pre-infection; 26.0 ± 2.0 pg/ml 7 days postinfection; p &lt; 0.001). INDO prevented this increase, so that infected-treated and control non-infected animals had similar levels of plasma PGE<sub>2</sub>. INDO reduced liver and abscess weight by 18% and 30% respectively (p &lt; 0.05). Cyclooxygenase (COX) activity determination by thin layer chromatography using (1-<sup>14</sup>C) arachidonic acid (AA) showed that liver microsomes from infected animals produced more PGE<sub>2</sub> than controls. COX activity was considerably inhibited in infected INDO-treated animals. Our data suggest that E. histolytica can stimulate the hepatic production of PGE<sub>2</sub> which contributes to pathogenesis of amoebic abscesses through generation and support of the inflammation. The partial effect of INDO treatment suggests that additional factors are involved.</p></div>\",\"PeriodicalId\":20653,\"journal\":{\"name\":\"Prostaglandins\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1997-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0090-6980(97)00058-0\",\"citationCount\":\"27\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Prostaglandins\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0090698097000580\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Prostaglandins","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0090698097000580","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 27

摘要

溶组织内阿米巴可以通过前列腺素E2 (PGE2)机制调节巨噬细胞功能和细胞因子的产生。为了研究PGE2在阿米巴肝脓肿形成中的作用,我们测试了PG合成抑制剂吲哚美辛(indomethacin, INDO)对肝内溶组织芽胞杆菌感染的仓鼠肝脓肿形成的影响。雄性感染动物感染前血浆PGE2水平较高(5.7±0.7 pg/ml);感染后7天26.0±2.0 pg/ml;p & lt;0.001)。INDO阻止了这种增加,因此接受治疗的感染动物和对照的未感染动物的血浆PGE2水平相似。INDO分别使肝脏和脓肿重量减轻18%和30% (p <0.05)。用(1-14C)花生四烯酸(AA)薄层色谱法测定环氧化酶(COX)活性表明,感染动物肝微粒体产生的PGE2比对照组多。在受感染的indo处理的动物中,COX活性明显受到抑制。我们的数据表明,溶组织杆菌可以刺激肝脏产生PGE2, PGE2通过产生和支持炎症参与阿米巴脓肿的发病机制。INDO治疗的部分效果表明还涉及其他因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of Prostaglandin E2 on Amoebic Liver Abscess Formation in Hamsters

Entamoeba histolytica can modulate macrophage functions and cytokine production by a prostaglandin E2 (PGE2) mechanism. To study the participation of PGE2 on amoebic liver abscess formation, we tested the effect of the PG synthesis inhibitor indomethacin (INDO) on abscess development in hamsters infected intrahepatically with E. histolytica trophozoites. Male infected animals had higher levels of plasma PGE2 (5.7 ± 0.7 pg/ml pre-infection; 26.0 ± 2.0 pg/ml 7 days postinfection; p < 0.001). INDO prevented this increase, so that infected-treated and control non-infected animals had similar levels of plasma PGE2. INDO reduced liver and abscess weight by 18% and 30% respectively (p < 0.05). Cyclooxygenase (COX) activity determination by thin layer chromatography using (1-14C) arachidonic acid (AA) showed that liver microsomes from infected animals produced more PGE2 than controls. COX activity was considerably inhibited in infected INDO-treated animals. Our data suggest that E. histolytica can stimulate the hepatic production of PGE2 which contributes to pathogenesis of amoebic abscesses through generation and support of the inflammation. The partial effect of INDO treatment suggests that additional factors are involved.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信