Percoll梯度离心法分离瓦登海沉积物中的硅藻硅藻产量、物种恢复和种群多样性。

Microbiologia (Madrid, Spain) Pub Date : 1997-06-01
S Sevecke, T Morawietz, W E Krumbein, E Rhiel
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引用次数: 0

摘要

采用Percoll梯度离心技术对来自瓦登海沿岸Dangast(德国湾)附近的沉积物样品进行了分析。实验还对瓦登海沉积物接种硅藻、无菌硅藻和混合硅藻培养物进行了实验。对于无菌和混合培养,总回收率在梯度上的硅藻的6 - 70%之间,而在瓦登海沉积物的大多数实验中,产量低于5%。从混合培养物和沉积物样品中提取硅藻不是定量的,无论是在生物多样性方面还是在提取的总个体方面,因为不同物种的恢复率各不相同。Percoll梯度离心法提取的硅藻细胞无沙粒和碎屑。所得材料适合用于生化实验、培养研究和电子显微镜。整体细胞形态和亚细胞细胞器组织良好,似乎不受纯化过程的影响。讨论了该技术的效果和优缺点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of Percoll gradient centrifugation for the isolation of diatoms from Wadden Sea sediments; diatom yields, species recoveries and population diversity.

The technique of Percoll gradient centrifugation was applied to samples from Wadden Sea sediments of the shoreside near Dangast (German Bight). Experiments were also performed with Wadden Sea sediment inoculated with diatoms and with axenic and mixed diatom cultures. For axenic and mixed cultures, total recoveries ranged from 6 to 70% of the diatoms loaded onto the gradients, whereas the yields were less than 5% in most of the experiments with Wadden Sea sediments. The extraction of diatoms from mixed cultures and from sediment samples was not quantitative, neither in terms of biodiversity nor of total individuals extracted, because the rates of recovery varied for the different species. Diatom cells extracted by Percoll gradient centrifugation were devoid of sand grains and debris. The obtained material was suitable to be used in biochemical experiments, in cultural studies and for electron microscopy. The overall cell morphology and subcellular organelle organization was excellent and appeared to be unaffected by the purification procedure. The results and advantages and disadvantages of the technique are discussed.

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