{"title":"增加ipa基因表达的福氏志贺氏菌criR基因的鉴定:双组分信号转导家族反应调节因子的新成员。","authors":"M S Qi, H Yoshikura, H Watanabe","doi":"10.7883/yoken1952.49.219","DOIUrl":null,"url":null,"abstract":"<p><p>A genetic locus named cri, which enhanced the expression of ipa genes, was cloned into Escherichia coli K-12 from Shigella flexneri 1b chromosomal DNA. Subcloning and Tn5-Tc1 transposon experiments showed that cri locus was located on a 2.6-kb HindIII fragment. Nucleotide sequence analysis of the region revealed at least three open reading frames (ORF), one of which, named criR, encoded a protein of 226 amino-acid residues and transcriptionally increased the ipaB expression. The deduced regulatory protein CriR shared a significant homology with bacterial transcriptional activators of the two-component signal transduction family. A homologue of the criR gene was present in genomic DNA of Shigella spp. and E. coli strains, and mapped at the 14.6-min region of E. coli K-12 chromosomal DNA. These results indicate that criR is a new member of response regulators.</p>","PeriodicalId":14531,"journal":{"name":"Japanese journal of medical science & biology","volume":"49 5-6","pages":"219-39"},"PeriodicalIF":0.0000,"publicationDate":"1996-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"Identification of a Shigella flexneri criR gene increasing ipa genes expression: a novel member of response regulators of the two-component signal transduction family.\",\"authors\":\"M S Qi, H Yoshikura, H Watanabe\",\"doi\":\"10.7883/yoken1952.49.219\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>A genetic locus named cri, which enhanced the expression of ipa genes, was cloned into Escherichia coli K-12 from Shigella flexneri 1b chromosomal DNA. Subcloning and Tn5-Tc1 transposon experiments showed that cri locus was located on a 2.6-kb HindIII fragment. Nucleotide sequence analysis of the region revealed at least three open reading frames (ORF), one of which, named criR, encoded a protein of 226 amino-acid residues and transcriptionally increased the ipaB expression. The deduced regulatory protein CriR shared a significant homology with bacterial transcriptional activators of the two-component signal transduction family. A homologue of the criR gene was present in genomic DNA of Shigella spp. and E. coli strains, and mapped at the 14.6-min region of E. coli K-12 chromosomal DNA. These results indicate that criR is a new member of response regulators.</p>\",\"PeriodicalId\":14531,\"journal\":{\"name\":\"Japanese journal of medical science & biology\",\"volume\":\"49 5-6\",\"pages\":\"219-39\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Japanese journal of medical science & biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7883/yoken1952.49.219\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese journal of medical science & biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7883/yoken1952.49.219","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Identification of a Shigella flexneri criR gene increasing ipa genes expression: a novel member of response regulators of the two-component signal transduction family.
A genetic locus named cri, which enhanced the expression of ipa genes, was cloned into Escherichia coli K-12 from Shigella flexneri 1b chromosomal DNA. Subcloning and Tn5-Tc1 transposon experiments showed that cri locus was located on a 2.6-kb HindIII fragment. Nucleotide sequence analysis of the region revealed at least three open reading frames (ORF), one of which, named criR, encoded a protein of 226 amino-acid residues and transcriptionally increased the ipaB expression. The deduced regulatory protein CriR shared a significant homology with bacterial transcriptional activators of the two-component signal transduction family. A homologue of the criR gene was present in genomic DNA of Shigella spp. and E. coli strains, and mapped at the 14.6-min region of E. coli K-12 chromosomal DNA. These results indicate that criR is a new member of response regulators.
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