多磷酸激酶的表达抑制了大肠杆菌的葡萄糖摄取。

J K Liu, M W Lu, L T Yang, C S Lin
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引用次数: 0

摘要

本文通过在lac启动子调控下表达多磷酸激酶,探讨了磷酸池和多磷酸激酶的表达对葡萄糖摄取的影响。在大肠杆菌中转化含有IPTG可控元件的pL1,发现细胞内磷酸盐总量显著增加。然而,IPTG诱导的静息质粒携带细胞的葡萄糖摄取率显著降低。这些发现表明,聚磷酸盐不能直接作为大肠杆菌的能量来源,或者至少不是一个好的能量供应者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Expression of polyphosphate kinase inhibits the glucose uptake in Escherichia coli.

This paper examines the effects of phosphate pool and expression of polyphosphate kinase on glucose uptake by expressing the polyphosphate kinase under the control of lac promoter. The E. coli transformant of pL1, containing an IPTG controllable element for polyphosphate kinase expression, showed that the total intracellular phosphate significantly increased. However, the rate of glucose uptake by the resting plasmid-bearing cells with IPTG induction significantly decreased. These findings suggest that the polyphosphate can not directly function as an energy source in E. coli or at least not as a good energy supplier.

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