S Okada, A Kitajima, T Tanimoto, H Suzuki, M Satake
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引用次数: 0
摘要
制备了“番泻皂苷参比标准品(对照品951)”,用于“番泻皂苷”制剂中番泻皂苷含量的荧光光度测定。在本试验中,羟基化的单蒽醌和二蒽醌糖苷与硼酸螯合,并根据参考标准(RS)测定螯合物的荧光强度。在本标准体系的建立中,必须用荧光光度法准确测定候选物质中sen皂苷的含量。用纯化的Sennoside A和Sennoside B的等摩尔混合物制备用于测定的Sennoside AB作为荧光光度法的RS。基于上述概念,通过荧光光度法测定候选物中sen皂苷为钙盐的含量为60.1 +/- 1.6%。经鉴定,该RS中sennosides的含量为60%。分别用高效液相色谱法测定该候选物中Sennoside A (SA)和Sennoside B (SB)的含量。结果表明,SA和SB的游离酸之和约为38%。由此推测,除SA、SB和蒽醌类苷外,约20%的二蒽醌类苷可能以游离酸的形式存在于该Sennoside RS中。并对其分析结果进行了讨论,并与现行的参皂苷RS进行了比较。
[Sennosides Reference Standard (Control 951) of the National Institute of Health Sciences].
The "Sennosides Reference Standard (Control 951)" was prepared, which is intended to be used for the fluorophotometric assay of sennosides content in the preparation of "Sennosides". In this assay hydroxylated mono- and dianthraquinone glucosides are chelated with boric acid, and the fluorescence intensity of the chelate is determined against that of the Reference Standard (RS). In the establishment of this RS, sennosides content in the candidate material must be determined accurately by fluorophotometry. The Sennoside AB for assay, prepared as an equimolar mixture of the purified sennoside A and Sennoside B, was used as the RS for the fluorophotometry. Based on the above concept, sennosides content in the candidate was determined as calcium salts to be 60.1 +/- 1.6% by the fluorophotometry. Thus the sennosides content of this Sennosides RS was certified to be 60%. Separately, contents of Sennoside A (SA) and Sennoside B (SB) in this candidate were determined by using HPLC. As a result, the sum of SA and SB was estimated to be 38% as free acids. Thus it was suggested that about 20% of dianthraquinone glucosides other than SA and SB and anthraquinone glucosides may be included in this Sennoside RS as free acids. Analytical results on the USP Sennosides RS were also shown and discussed, compared with the present Sennosides RS.