{"title":"内皮素通过ETB受体亚型收缩小鼠肝小静脉和肝窦。","authors":"Y Ito, M Katori, M Majima, A Kakita","doi":"10.1159/000179181","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to examine the effects of endothelin (ET)-1 and ET-3 on hepatic venules and sinusoids and to identify the subtypes of ET receptors.</p><p><strong>Method: </strong>Hepatic venules and sinusoids of anesthetized mice were observed at the edge of the liver. ET-1, ET-3 and sarafotoxin (S6c, a selective ETB receptor agonist) were applied topically over the microvasculature.</p><p><strong>Results: </strong>ET-1, ET-3 and S6c (1-100 microM, 30 microliters) induced dose-dependent vasoconstriction of the portal venules, the sinusoids and the central venules. The ETs and S6c were equipotent for these microvessels. BQ-123 (a selective ETA receptor antagonist) inhibited the constrictive effects of ET-3 (not of ET-1) on the portal venules and central venules, whereas it had no inhibitory effect on the sinusoids.</p><p><strong>Conclusions: </strong>In mouse hepatic venules and sinusoids, the vasoconstriction induced by ET-1 and ET-3 was mediated mainly through the ETB receptor subtype and partly through an unknown BQ-123-sensitive ET receptor subtype in the portal and central venules, and only through the ETB receptor subtype in the sinusoids.</p>","PeriodicalId":14035,"journal":{"name":"International journal of microcirculation, clinical and experimental","volume":"16 5","pages":"250-8"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000179181","citationCount":"14","resultStr":"{\"title\":\"Constriction of mouse hepatic venules and sinusoids by endothelins through ETB receptor subtype.\",\"authors\":\"Y Ito, M Katori, M Majima, A Kakita\",\"doi\":\"10.1159/000179181\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>This study was conducted to examine the effects of endothelin (ET)-1 and ET-3 on hepatic venules and sinusoids and to identify the subtypes of ET receptors.</p><p><strong>Method: </strong>Hepatic venules and sinusoids of anesthetized mice were observed at the edge of the liver. ET-1, ET-3 and sarafotoxin (S6c, a selective ETB receptor agonist) were applied topically over the microvasculature.</p><p><strong>Results: </strong>ET-1, ET-3 and S6c (1-100 microM, 30 microliters) induced dose-dependent vasoconstriction of the portal venules, the sinusoids and the central venules. The ETs and S6c were equipotent for these microvessels. BQ-123 (a selective ETA receptor antagonist) inhibited the constrictive effects of ET-3 (not of ET-1) on the portal venules and central venules, whereas it had no inhibitory effect on the sinusoids.</p><p><strong>Conclusions: </strong>In mouse hepatic venules and sinusoids, the vasoconstriction induced by ET-1 and ET-3 was mediated mainly through the ETB receptor subtype and partly through an unknown BQ-123-sensitive ET receptor subtype in the portal and central venules, and only through the ETB receptor subtype in the sinusoids.</p>\",\"PeriodicalId\":14035,\"journal\":{\"name\":\"International journal of microcirculation, clinical and experimental\",\"volume\":\"16 5\",\"pages\":\"250-8\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1159/000179181\",\"citationCount\":\"14\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of microcirculation, clinical and experimental\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1159/000179181\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of microcirculation, clinical and experimental","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1159/000179181","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
摘要
目的:探讨内皮素(ET)-1和ET-3对肝小静脉和肝窦的影响,并鉴定ET受体的亚型。方法:麻醉小鼠肝边缘观察肝小静脉和肝窦。ET-1, ET-3和sarafotoxin (S6c,一种选择性的ETB受体激动剂)局部应用于微血管。结果:ET-1、ET-3和S6c (1 ~ 100 μ m, 30微升)诱导门静脉、窦状静脉和中央静脉呈剂量依赖性收缩。ETs和S6c对这些微血管具有同等效力。BQ-123(一种选择性ETA受体拮抗剂)抑制ET-3(非ET-1)对门静脉和中央小静脉的收缩作用,而对窦状静脉没有抑制作用。结论:在小鼠肝小静脉和肝窦中,ET-1和ET-3诱导的血管收缩主要通过ETB受体亚型介导,部分通过门脉和中央小静脉中未知的bq -123敏感ET受体亚型介导,而仅通过ETB受体亚型介导。
Constriction of mouse hepatic venules and sinusoids by endothelins through ETB receptor subtype.
Objective: This study was conducted to examine the effects of endothelin (ET)-1 and ET-3 on hepatic venules and sinusoids and to identify the subtypes of ET receptors.
Method: Hepatic venules and sinusoids of anesthetized mice were observed at the edge of the liver. ET-1, ET-3 and sarafotoxin (S6c, a selective ETB receptor agonist) were applied topically over the microvasculature.
Results: ET-1, ET-3 and S6c (1-100 microM, 30 microliters) induced dose-dependent vasoconstriction of the portal venules, the sinusoids and the central venules. The ETs and S6c were equipotent for these microvessels. BQ-123 (a selective ETA receptor antagonist) inhibited the constrictive effects of ET-3 (not of ET-1) on the portal venules and central venules, whereas it had no inhibitory effect on the sinusoids.
Conclusions: In mouse hepatic venules and sinusoids, the vasoconstriction induced by ET-1 and ET-3 was mediated mainly through the ETB receptor subtype and partly through an unknown BQ-123-sensitive ET receptor subtype in the portal and central venules, and only through the ETB receptor subtype in the sinusoids.