[肿瘤坏死因子α调节颗粒细胞增殖活性和分化功能:猪模型体外研究]。

Nihon Sanka Fujinka Gakkai zasshi Pub Date : 1996-11-01
J Deguchi, T Maruo, H Matsuo, M Mochizuki
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引用次数: 0

摘要

卵巢卵泡发生是卵泡生长分化的动态过程。现在很明显,各种生长因子与FSH相互作用,改变卵泡的生长和功能。本研究采用猪模型,在体外研究了肿瘤坏死因子α (TNF α)对颗粒细胞增殖潜能和类固醇生成能力的影响,并将其作为滤泡生长的功能。从小(1-2mm)、中(3-5mm)和大(6-12 mm)卵泡中获得的猪颗粒细胞在无血清条件下,在存在或不存在FSH (100 ng/ml)和IGF-1 (100 ng/ml)的情况下,在有或没有不同浓度的TNF α的情况下培养。通过增殖细胞核抗原单克隆抗体(PCNA)和[3H]胸苷摄取免疫细胞化学技术评估培养颗粒细胞的增殖活性,通过测定颗粒细胞分泌黄体酮和17 β -雌二醇的能力来评估颗粒细胞的分化功能。FSH和igf - 1的加入增强了培养颗粒细胞的增殖活性和类固醇生成能力。在颗粒细胞培养中,FSH和IGF-I处理引起的增殖活性和类固醇生成能力的增加被TNF - α同时处理显著降低。TNF α对小卵泡颗粒细胞增殖活性的抑制作用明显,而对大卵泡颗粒细胞雌二醇分泌的抑制作用非常强。TNF α对黄体酮分泌的抑制作用与卵泡生长阶段无关。这些结果提示TNF - α参与调节颗粒细胞的增殖和分化。在卵泡生长过程中,TNF α对颗粒细胞的生物学作用可能从抑制未成熟卵泡的增殖活性转变为抑制成熟卵泡的分化功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Tumor necrosis factor alpha regulates the proliferative activity and differentiated function of granulosa cells: in vitro study with a porcine model].

Ovarian folliculogenesis is a dynamic process during which follicles undergo growth and differentiation. It is now evident that various growth factors interact with FSH to modify follicular growth and function. In the present study, the effects of tumor necrosis factor alpha (TNF alpha) on the proliferative potential and steroidogenic ability of granulosa cells were examined in vitro as a function of follicular growth by using a porcine model. Porcine granulosa cells obtained from small (1-2mm), medium (3-5mm) and large (6-12 mm) follicles were cultured under serum-free conditions in the presence or absence of FSH (100 ng/ml) and IGF-1 (100 ng/ml), with or without various concentrations of TNF alpha. The proliferative activity of cultured granulosa cells was assessed by immunocytochemical techniques with a monoclonal antibody to proliferating cell nuclear antigen (PCNA) and by [3H]-thymidine uptake, while differentiated functions of granulosa cells were assessed by determining the ability to secrete progesterone and 17 beta-estradiol. The addition of FSH and IGF-I augmented the proliferative activity and steroidogenic ability of cultured granulosa cells. The increases in proliferative activity and steroidogenic ability caused by treatment with FSH and IGF-I were significantly reduced by the concomitant treatment with TNF alpha in culture of granulosa cells. The inhibitory effect of TNF alpha on the proliferative activity was prominent in small follicle granulosa cells, whereas the inhibitory effect of TNF alpha on estradiol secretion was very strong in large follicle granulosa cells. The inhibitory effect of TNF alpha on progesterone secretion was apparent regardless of the stage of follicular growth. These results suggest TNF alpha participation in regulating the proliferation and differentiation of granulosa cells. In appears that the biological action of TNF alpha on granulosa cells may shift from the inhibition of proliferative activity in immature follicles to the inhibition of differentiated function in mature follicles during the course of follicular growth.

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