M Yasue, Y Akasaki, T R Numoto, S Abe, T Abe, Y Takeuchi, J Tanaka
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引用次数: 0
摘要
探讨脑膜瘤S期和G2/ m期分数与mb -1指数的相关性。采用改进的Hedley's方法，对29例脑膜瘤28例和血管外皮细胞瘤1例的81份石蜡包埋组织样本的细胞悬液进行了流式细胞术分析。微波处理后石蜡包埋组织切片用抗mib -1单克隆抗体染色。对500个细胞进行了评分。使用线性回归估计数据之间的相关性。4/29的肿瘤存在DNA非整倍体。25例非复发肿瘤的平均S期和G2/ m期评分分别为1.77和4.76%，复发肿瘤的平均S期和G2/ m期评分分别为2.41和5.81%。非复发肿瘤的增殖指数(PI = S + G2/M分数)为6.52%，复发肿瘤为8.29%。非复发肿瘤的平均MIB-1评分为1.25%，复发肿瘤的平均MIB-1评分为2.69%。s相分数或PI百分比与MIB-1评分呈线性相关。这两种方法都有助于评估脑膜瘤的增殖活性。
MIB-1 immunostaining and DNA flow cytometry in meningiomas.
The correlation between S- and G2/M-phase fractions and MIB-1 index in meningiomas was investigated. Flow cytometric analyses were performed on cell suspensions from 81 samples of paraffin-embedded tissue from 29 patients with a total of 28 meningiomas and 1 hemangiopericytoma using the modified Hedley's method. Sections from the paraffin-embedded tissues were stained with anti-MIB-1 monoclonal antibody after microwave oven processing. Five hundreds cells were scored. Correlations between data were estimated using linear regression. DNA aneuploidy was present in 4/29 tumors. Mean S- and G2/M-phase fractions in the 25 nonrecurrent tumors were 1.77 and 4.76%, and in the recurrent tumors were 2.41 and 5.81%, respectively. The proliferative index (PI = S + G2/M fraction) was 6.52% in the nonrecurrent tumors and 8.29% in the recurrent tumors. The mean MIB-1 score in the nonrecurrent tumors was 1.25% and in the recurrent tumors was 2.69%. There was a linear correlation between percentage of S-phase fraction or PI and the MIB-1 score. Both methods are useful to assess the proliferative activity in meningioma.
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