肌酸激酶活性随短期冷冻而降低。

Enzyme & protein Pub Date : 1994-01-01 DOI:10.1159/000474994
E I Lev, I Hendler, R Siebner, Z Tashma, M Wiener, I Tur-Kaspa
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引用次数: 12

摘要

在-30℃下不加保护剂将血清样品冷冻是血清库中最简单、最便宜的储存方法。我们研究了在这种条件下冷冻保存24小时(n = 30)或2或4周(n = 99)后,人血清中肌酸激酶(CK)的稳定性。测定了新鲜血清中的CK活性,并在指定的时间间隔内与冷冻后的匹配解冻血清进行了比较。冷冻24小时、2周和4周后,酶的中位活性分别显著降低了2.6、5.9和8.3% (p < 0.0001, r = 0.99)。性别或高CK初始值对这些结果没有显著影响。我们得出结论,血清在-30℃的冷冻储存,即使是很短的时间,也会导致CK活性的稳定和显著下降。在分析冷冻血清中的CK活性用于研究或临床目的时,应考虑到这些结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Creatine kinase activity decrease with short-term freezing.

Freezing of serum samples at -30 degrees C without protective agents is the simplest and least expensive method of storage in serum banks. We investigated the stability of creatine kinase (CK) in human sera after freezer storage under such conditions for 24 h (n = 30) or for 2 or 4 weeks (n = 99). CK activity was measured in fresh sera and compared to matched thawed sera after freezer storage at the designated time intervals. The enzyme's median activity decreased significantly after 24 h, 2 weeks, and 4 weeks of freezer storage by 2.6, 5.9, and 8.3%, respectively (p < 0.0001, r = 0.99). Sex or high CK initial values had no significant effect on these results. We conclude that freezer storage of serum at -30 degrees C, even for short periods, causes a steady and significant decline in CK activity. These results should be taken into consideration when analyzing CK activity in frozen sera for research or clinical purposes.

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