{"title":"DNA测序中的荧光光漂白","authors":"Benjamin Chu","doi":"10.1016/0065-227X(96)84739-3","DOIUrl":null,"url":null,"abstract":"<div><p>Advances in DNA-sequencing technology suggest many possibilities using either conventional or unconventional means. Several approaches offer conceivable improvements in running time by one hundred fold or more. A fluorescence photobleaching scheme in combination with ultra-thin gel slab or capillary electrophoresis which proposes to shorten the run time and to increase the resolution of multi-fragment DNA analysis is reviewed. The advantages and some of the yet to be resolved obstacles are discussed.</p></div>","PeriodicalId":50880,"journal":{"name":"Advances in Biophysics","volume":"32 ","pages":"Pages 17-29"},"PeriodicalIF":0.0000,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0065-227X(96)84739-3","citationCount":"0","resultStr":"{\"title\":\"Fluorescence photobleaching in DNA sequencing\",\"authors\":\"Benjamin Chu\",\"doi\":\"10.1016/0065-227X(96)84739-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Advances in DNA-sequencing technology suggest many possibilities using either conventional or unconventional means. Several approaches offer conceivable improvements in running time by one hundred fold or more. A fluorescence photobleaching scheme in combination with ultra-thin gel slab or capillary electrophoresis which proposes to shorten the run time and to increase the resolution of multi-fragment DNA analysis is reviewed. The advantages and some of the yet to be resolved obstacles are discussed.</p></div>\",\"PeriodicalId\":50880,\"journal\":{\"name\":\"Advances in Biophysics\",\"volume\":\"32 \",\"pages\":\"Pages 17-29\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0065-227X(96)84739-3\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advances in Biophysics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0065227X96847393\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Biophysics","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0065227X96847393","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Advances in DNA-sequencing technology suggest many possibilities using either conventional or unconventional means. Several approaches offer conceivable improvements in running time by one hundred fold or more. A fluorescence photobleaching scheme in combination with ultra-thin gel slab or capillary electrophoresis which proposes to shorten the run time and to increase the resolution of multi-fragment DNA analysis is reviewed. The advantages and some of the yet to be resolved obstacles are discussed.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
