{"title":"大鼠巨核细胞Ca2+振荡机制的药理学研究","authors":"Chikako Uneyama , Hisayuki Uneyama , Michihito Takahashi , Norio Akaike","doi":"10.1016/0922-4106(95)90080-2","DOIUrl":null,"url":null,"abstract":"<div><p>Extracellular application of ATP evoked the oscillatory K<sup>+</sup> currents (<em>I</em><sub>KCa</sub>) reflecting oscillation in cytoplasmic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) of megakaryocyte isolated from rat bone marrow. We have reported that the [Ca<sup>2+</sup>]<sub>i</sub> oscillation was regulated by intracellular Ca<sup>2+</sup>-pumping activity (Uneyama, H., C. Uneyama and N. Akaike, 1993, J. Biol. Chem. 268, 168). Here we found that the Ca<sup>2+</sup> pump of the megakaryocyte could be divided into at least two classes according to the sensitivity to phosphorylation-modulating drugs. The effects of protein kinase C and cyclic AMP-dependent protein kinase are complementary, and the effect of Ca<sup>2+</sup>/calmodulin is independent of the above two kinases. In addition, this is the first report concerning the physiological regulation of Ca<sup>2+</sup>-ATPase in living cells.</p></div>","PeriodicalId":100502,"journal":{"name":"European Journal of Pharmacology: Molecular Pharmacology","volume":"291 3","pages":"Pages 381-386"},"PeriodicalIF":0.0000,"publicationDate":"1995-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0922-4106(95)90080-2","citationCount":"3","resultStr":"{\"title\":\"Pharmacological studies on mechanisms involved in Ca2+ oscillations in rat megakaryocytes\",\"authors\":\"Chikako Uneyama , Hisayuki Uneyama , Michihito Takahashi , Norio Akaike\",\"doi\":\"10.1016/0922-4106(95)90080-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Extracellular application of ATP evoked the oscillatory K<sup>+</sup> currents (<em>I</em><sub>KCa</sub>) reflecting oscillation in cytoplasmic Ca<sup>2+</sup> concentration ([Ca<sup>2+</sup>]<sub>i</sub>) of megakaryocyte isolated from rat bone marrow. We have reported that the [Ca<sup>2+</sup>]<sub>i</sub> oscillation was regulated by intracellular Ca<sup>2+</sup>-pumping activity (Uneyama, H., C. Uneyama and N. Akaike, 1993, J. Biol. Chem. 268, 168). Here we found that the Ca<sup>2+</sup> pump of the megakaryocyte could be divided into at least two classes according to the sensitivity to phosphorylation-modulating drugs. The effects of protein kinase C and cyclic AMP-dependent protein kinase are complementary, and the effect of Ca<sup>2+</sup>/calmodulin is independent of the above two kinases. In addition, this is the first report concerning the physiological regulation of Ca<sup>2+</sup>-ATPase in living cells.</p></div>\",\"PeriodicalId\":100502,\"journal\":{\"name\":\"European Journal of Pharmacology: Molecular Pharmacology\",\"volume\":\"291 3\",\"pages\":\"Pages 381-386\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-11-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0922-4106(95)90080-2\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"European Journal of Pharmacology: Molecular Pharmacology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0922410695900802\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"European Journal of Pharmacology: Molecular Pharmacology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0922410695900802","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
摘要
细胞外应用ATP引起振荡K+电流(IKCa)，反映了从大鼠骨髓分离的巨核细胞胞质Ca2+浓度([Ca2+]i)的振荡。我们已经报道了细胞内Ca2+泵送活动调节[Ca2+]i振荡(Uneyama, H.， C. Uneyama and N. Akaike, 1993, J. Biol.)。化学。268,168)。这里我们发现，根据对磷酸化调节药物的敏感性，巨核细胞的Ca2+泵可以分为至少两类。蛋白激酶C和环amp依赖性蛋白激酶的作用是互补的，Ca2+/钙调蛋白的作用独立于上述两种激酶。此外，这是关于Ca2+- atp酶在活细胞中的生理调节的第一篇报道。
Pharmacological studies on mechanisms involved in Ca2+ oscillations in rat megakaryocytes
Extracellular application of ATP evoked the oscillatory K+ currents (IKCa) reflecting oscillation in cytoplasmic Ca2+ concentration ([Ca2+]i) of megakaryocyte isolated from rat bone marrow. We have reported that the [Ca2+]i oscillation was regulated by intracellular Ca2+-pumping activity (Uneyama, H., C. Uneyama and N. Akaike, 1993, J. Biol. Chem. 268, 168). Here we found that the Ca2+ pump of the megakaryocyte could be divided into at least two classes according to the sensitivity to phosphorylation-modulating drugs. The effects of protein kinase C and cyclic AMP-dependent protein kinase are complementary, and the effect of Ca2+/calmodulin is independent of the above two kinases. In addition, this is the first report concerning the physiological regulation of Ca2+-ATPase in living cells.
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