MeIQ在Big Blue®lacI转基因小鼠中致突变性的器官变异

Mutation Research/Genetic Toxicology Pub Date : 1996-07-10 DOI:10.1016/S0165-1218(96)90046-4

Takayoshi Suzuki , Makoto Hayashi , Masako Ochiai , Keiji Wakabayashi , Toshikazu Ushijima , Takashi Sugimura , Minako Nagao , Toshio Sofuni

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引用次数: 55

摘要

2-氨基-3,4-二甲基咪唑[4,5-f]喹啉(MeIQ)是一种存在于食物中的杂环胺，也是鼠伤寒沙门氏菌TA98的有效诱变剂，我们利用lacI转基因小鼠(Big Blue®，C57BL/6N系)研究了其潜在的遗传毒性。7周龄雌性小鼠分别饲喂含0.03% MeIQ的日粮1、4和12周，分析骨髓、肝脏、前胃、结肠和心脏的突变频率(MF)。MF呈饲养周期依赖性增加。与未治疗小鼠相比，MeIQ喂养12周后，小鼠结肠的MF高38倍，骨髓的MF高5.8倍，肝脏的MF高4.6倍，前胃的MF高2.6倍。在心脏中未检测到MF增加，没有肿瘤发生。

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Organ variation in the mutagenicity of MeIQ in Big Blue® lacI transgenic mice

2-Amino-3,4-dimethylimidazo[4,5-f]quinoline (MeIQ), which is a heterocyclic amine found in food and the potent mutagen in S. typhimurium TA98, was examined for its genotoxic potential using lacI transgenic mice (Big Blue®, C57BL/6N lineage). Female mice, at 7 weeks of age, were given a diet containing 0.03% MeIQ for 1, 4 and 12 weeks, and mutant frequencies (MF) were analyzed in the bone marrow, liver, forestomach, colon and heart. The MF increased in a feeding period-dependent manner. Relative to untreated mice, the MF after a 12-week-feeding of MeIQ was 38 times higher in the colon, 5.8 times higher in the bone marrow, 4.6 times higher in the liver, and 2.6 times higher in the forestomach. No increase in MF was detected in the heart, where no tumors develop.

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Mutation Research/Genetic Toxicology

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