T Nomura, M Ohtsuki, S Matsui, C Sumi-Ichinose, H Nomura, Y Hagino, K Iwase, H Ichinose, K Fujita, T Nagatsu
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引用次数: 7
摘要
虽然已经报道了人类GTP环水解酶I (GCH I)的三种不同的cDNA形式的存在(Togari et al.， 1992)，但任何涉及多(A)尾的人类GCH I cDNA的全长序列尚未被记录。本研究首先从人嗜铬细胞瘤cDNA文库中分离出编码人GCHⅰ型1的全长cDNA克隆。全长2921个碱基对(含poly (A) tail)。RNA印迹分析显示，嗜铬细胞瘤组织中存在一个4.0 kb的单一mRNA种。
Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma.
Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single mRNA species of 4.0 kb in human pheochromocytoma tissue.
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