去甲肾上腺素前体氨基酸l -三邻3,4-二羟基苯基丝氨酸在大脑中的新代谢途径。体内微透析研究。

W Maruyama, D Nakahara, M Naoi
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引用次数: 11

摘要

采用体内微透析的方法,研究了l -苏-3,4-二羟基苯基丝氨酸(l -苏- dops)在大鼠脑纹状体中的代谢及其作用。脑内L-threo-DOPS被3种不同的酶代谢;芳香l-氨基酸脱羧酶,儿茶酚- o -甲基转移酶和dops醛缩酶。dops醛缩酶是l -三o- dops代谢的主要酶。l- 3 - dops灌注100 min后,l-氨基酸脱羧酶(去甲肾上腺素及其代谢物)代谢物含量占透析液代谢物总量的0.4%,儿茶酚- o-甲基转移酶代谢物含量为2.1%,dops -醛缩酶代谢物含量为97.5%。L-threo-DOPS被发现可以增加细胞外多巴胺和血清素的水平,并抑制大脑中单胺的分解代谢。抑制dops醛缩酶可提高其作为去甲肾上腺素补充治疗的有效性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A new metabolic pathway of L-threo-3,4-dihydroxyphenylserine, a precursor amino acid of norepinephrine, in the brain. Studies by in vivo microdialysis.

The metabolism and the effects of L-threo-3,4-dihydroxyphenylserine (L-threo-DOPS) were studied in the rat brain striatum by in vivo microdialysis. In the brain L-threo-DOPS was metabolized by 3 different enzymes; aromatic L-amino acid decarboxylase, catechol-O-methyltransferase, and DOPS-aldolase. DOPS-aldolase was the main enzyme which metabolizes L-threo-DOPS. The amounts of the metabolites by L-amino acid decarboxylase (norepinephrine and its metabolites) were 0.4% of the total amounts of metabolites detected in the dialysate, while those by catechol-O-methyltransferase, 2.1%, and by DOPS-aldolase, 97.5%, after 100 min perfusion of L-threo-DOPS. L-threo-DOPS was found to increase extracellular levels of dopamine and serotonin, and to inhibit monoamine catabolism in the brain. Inhibition of DOPS-aldolase should improve its effectiveness as the supplement therapy of norepinephrine.

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