人淋巴细胞cDNA序列文库的二维凝胶电泳分析。3.个体克隆分析。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1995-01-01

G Béhar, C Coleclough, R Houlgatte, C Auffray, I Lefkovits

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引用次数: 0

摘要

我们的目标是通过结合基因数据和表达蛋白数据来获得淋巴细胞cDNA文库的全局信息。有序的文库使我们能够通过从矩阵(在我们的系统中是一个24 x 16 x 12的数组)中直接检索单个克隆来建立克隆的属性。我们对从文库中获得的39个克隆的x射线x光片进行了检查，并对细胞自由表达的克隆产物进行了2D凝胶分析，并确定了它们的部分核苷酸序列。报告了两个这样的克隆的详细情况;克隆CEM1001为新基因，克隆CEM1002为人延伸因子1- β基因。

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Human lymphocyte cDNA ordered library analyzed by 2D gel electrophoresis. 3. Analysis of individual clones.

We pursued the goal of obtaining global information on cDNA libraries from lymphocytes by combining data on the genes with data on the expressed proteins. Ordered libraries enabled us to establish the properties of the clones by direct retrieval of individual clones from the matrix (in our system a 24 x 16 x 12 array). We have picked-upon inspection of radiofluorographs obtained from the pools of the library--39 clones and performed a 2D gel analysis on the cell free expressed clonal products and have established also their partial nucleotide sequences. Details on two such clones are reported; clone CEM1001 is a new gene, while clone CEM1002 is a gene for human elongation factor 1-beta.

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Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society

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