用HepG2细胞作为模型系统进行免疫荧光研究,固定对过氧化物酶体结构保存的影响。

The Histochemical Journal Pub Date : 1995-08-01
M Schrader, E Baumgart, H D Fahimi
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引用次数: 0

摘要

免疫荧光技术已成为研究细胞培养中过氧化物酶体的重要工具。我们用这种方法研究了人肝母细胞瘤细胞系HepG2的过氧化物酶体。检测到过氧化物酶体形式的明显异质性。除了球形结构(约100 nm)和棒状结构(约300 nm)外,还发现了许多细长的、波动的管状结构(可达5微米)。进一步的观察表明,免疫荧光中过氧化物酶体形式的出现取决于所使用的固定程序。使用甲醇-丙酮(-20℃)或乙醇固定会产生球状颗粒的点状图案,而使用甲醛/Triton X-100固定会显示保存完好的小管和棒。这些观察结果对于研究过氧化物酶体的生物发生可能具有特别重要的意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of fixation on the preservation of peroxisomal structures for immunofluorescence studies using HepG2 cells as a model system.

The immunofluorescence technique has become an important tool for the investigation of peroxisomes in cell culture. We have used this method for the study of peroxisomes in the human hepatoblastoma cell line HepG2. A marked heterogeneity of peroxisomal forms was detected. Besides spherical (about 100 nm) and rod-shaped structures (about 300 nm) many elongated, undulating tubular forms (up to 5 microns) were found. Further observations indicate that the appearance of the peroxisomal forms in immunofluorescence is dependent on the fixation procedure used. Whereas the fixation with methanol-acetone (-20 degrees C) or ethanol results in a punctate pattern with spherical particles, the use of formaldehyde/Triton X-100 fixation shows well-preserved tubules and rods. These observations may be of special importance for studies on the biogenesis of peroxisomes.

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