生物磷灰石晶体结构特征及特性观察。8. 人牙釉质晶体融合的观察。

T Ichijo, Y Yamashita, T Terashima
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引用次数: 0

摘要

为了研究生物磷灰石晶体的基本结构特征和特征,我们利用透射电子显微镜,在近原子分辨率下观察了人类牙釉质、牙本质和骨晶体的超微结构,并通过晶体的横切面和纵切面显示了羟基磷灰石结构的构型。随后,根据作者对牙齿和骨骼超微结构的观察结果,使用相同的方法,我们已经能够直接检查人类牙齿和骨骼晶体中晶格缺陷的图像,如晶体中的点缺陷结构、线缺陷和面缺陷。在本报告中,我们描述了用同样的方法从人牙釉质晶体切片中获得的晶体融合图像。本研究使用的材料是新拔出的人类下第一磨牙的无龋牙釉质。用常规方法将材料的小立方体固定在戊二醛和四氧化二锇中,并包埋在环氧树脂中。超薄切片用金刚石刀切割,不脱钙。采用日立H- 800h和H-9000型透射电子显微镜,分别在200千伏和300千伏下工作。每个晶体在初始放大30万倍和最终放大1000万倍以上时被观察到。因此,我们能够确认相邻晶体之间的融合可能发生在人类牙釉质生命历史的某个时候。我们真诚地相信,本报告中所显示的电子显微照片是第一个以近原子分辨率显示人类牙釉质晶体中晶体融合的超微结构。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Observations on structural features and characteristics of biological apatite crystals. 8. Observation on fusion of human enamel crystals.

In a series of studies to investigate the basic structural features and characteristics of the biological apatite crystals, using a transmission electron microscope, we examined the ultrastructure of the human enamel, dentin, and bone crystals at near atomic resolution and showed the configuration of the hydroxyapatite structure through the cross and longitudinal sections of the crystals. Subsequently, based on the results of the observations by the authors of the ultrastructure of the tooth and bone, using the same approach, we have been able to directly examine the images of the lattice imperfections in the human tooth and bone crystals, such as the point defect structure, line defect, and face defect, in the crystals. In this report, we describe the images of the crystal fusion obtained by using the same approach from the sections of the human enamel crystals. The materials used for this study were the noncarious enamel from the freshly extracted human erupted lower first molars. The small cubes of the material were fixed in glutaraldehyde and osmium tetroxide and embedded in epoxy resin using the routine methods. The ultrathin sections were cut with a diamond knife without decalcification. The sections were examined with the HITACHI H-800 H and H-9000 type transmission electron microscopes operated at 200 kV and 300 kV. Each crystal was observed at an initial magnification of 300,000 times and at a final magnification of 10,000,000 times and over. We are, therefore, able to confirm that the fusion between the adjacent crystals can occur at some time during the life history of the human enamel. We sincerely believe that the electron micrographs shown in this report are the first to show the ultrastructures of the crystal fusion in the human enamel crystals at near atomic resolution.

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