配体结合实验时间过程的非线性回归分析。

R G Duggleby, M J Waters
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引用次数: 2

摘要

在生物条件下,激素和生长因子的结合和释放往往是相对缓慢的过程。因此,了解潜在的速率常数可能比了解平衡常数具有更大的生理学意义。在这里,我们展示了如何通过一个单一的结合时间过程来确定结合和释放的速率常数。这些速率常数的比值可以计算出结合常数。描述了一个非线性回归计算机程序,它简化了这些计算,并提供了所确定常数的估计和标准误差。以人生长激素与人生长激素结合蛋白的结合和羊催乳素与兔催乳素受体的结合为例说明了该方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nonlinear regression analysis of the time course of ligand binding experiments.

The binding and release of hormones and growth factors are often relatively slow processes under biological conditions. Consequently, a knowledge of the underlying rate constants may be of greater physiological relevance than the equilibrium constant. Here we show how, by following a single time course of binding, the rate constants for both binding and release can be determined. The ratio of these rate constants allows the binding constant to be calculated. A nonlinear regression computer program is described which facilitates these calculations and which provides estimates and standard errors of the constants determined. The method is illustrated by the binding of human growth hormone to the human growth hormone binding protein, and the binding of ovine prolactin to the rabbit prolactin receptor.

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