人生长激素中亮氨酸残基的特异氮-15标记。

Acta chemica Scandinavica (Copenhagen, Denmark : 1989) Pub Date : 1993-10-01 DOI:10.3891/acta.chem.scand.47-0990

T Christensen, J Petersen, C F Theisen, K Bjerregaard, S M Kristensen, J J Led

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引用次数: 1

摘要

利用15N标记的亮氨酸和需要亮氨酸的大肠杆菌菌株，通过重组DNA技术获得了在亮氨酸残基中特异性标记15N的生物合成人类生长激素(hGH)。结果表明，尽管可能存在少量转氨酶活性，但标记总体上是特异性的，并且该方法可以大大简化hGH的二维1H-15N相关NMR谱。

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Specific nitrogen-15 labelling of leucine residues in human growth hormone.

Biosynthetic human growth hormone (hGH) specifically 15N labelled in the leucine residues has been obtained by recombinant DNA technology, using 15N-labelled leucine and an E. coli strain that requires leucine. It is shown that, despite the possibility of minor transaminase activity, the labelling on the whole is specific, and that the two-dimensional 1H-15N correlation NMR spectra of hGH can be greatly simplified by this methodology.

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Acta chemica Scandinavica (Copenhagen, Denmark : 1989)

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