高胆固醇血症对大鼠肾毒性血清肾炎初期和慢性期的影响:局灶节段性肾小球硬化的发展,类似于动脉粥样硬化。

N Baba, T Shimokama, T Watanabe
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引用次数: 7

摘要

研究了高胆固醇血症对大鼠肾毒性血清(NTS)肾炎初期和慢性期的影响。高胆固醇血症大鼠在NTS肾炎初始阶段的损伤,在补充胆固醇的饮食中维持(2组),其特征是肾小球内脂滴液泡细胞(泡沫细胞)的节段性增强积聚,而标准饮食(1组)的大鼠肾脏仅显示轻微的毛细血管内细胞增殖。免疫电镜观察显示,2组大鼠泡沫细胞主要来源于巨噬细胞。第5-6天,2组的肾小球巨噬细胞数量(以每个肾小球中ed1阳性细胞的数量定义)显著高于1组(1组3.4 +/- 1.4,2组6.3 +/- 1.0;p < 0.01)和第21-28天(第1组为5.5 +/- 2.6,第2组为10.9 +/- 2.8;P < 0.01)。相比之下,两组ox19阳性t淋巴细胞和ox33阳性b淋巴细胞的数量相似。在NTS肾炎的慢性期第20周,对肾小球病变的半定量评估显示,与第1组相比,第2组的局灶节段性肾小球硬化(FSGS)更为严重(肾小球损伤评分:第1组为14 +/- 10,第2组为73 +/- 17;P < 0.01)。脂质和泡沫细胞的堆积无一例外地出现在第二组动物的硬化灶中。提示高胆固醇血症在NTS肾炎大鼠FSGS加速发展中起重要作用。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of hypercholesterolemia on initial and chronic phases of rat nephrotoxic serum nephritis: development of focal segmental glomerulosclerosis, analogous to atherosclerosis.

The effects of hypercholesterolemia on both the initial and chronic phases of rat nephrotoxic serum (NTS) nephritis have been investigated. Injury during the initial phase of NTS nephritis in hypercholesterolemic rats maintained on a cholesterol-supplemented diet (Group 2) was characterized by segmentally accentuated accumulations of vacuolated cells with lipid droplets (foam cells) in the glomeruli, while the kidneys of rats fed a standard diet (Group 1) revealed only mild intracapillary cell proliferation. Immunoelectron microscopy showed that the foam cells observed in Group 2 rats were largely derived from macrophages. The glomerular macrophage number, defined by the number of ED1-positive cells per glomerulus, was significantly higher in Group 2 than in Group 1 animals at days 5-6 (3.4 +/- 1.4 in Group 1 against 6.3 +/- 1.0 in Group 2; p < 0.01) as well as at days 21-28 (5.5 +/- 2.6 in Group 1 against 10.9 +/- 2.8 in Group 2; p < 0.01). In contrast, the numbers of OX19-positive T-lymphocytes and OX33-positive B-lymphocytes were similar in both groups. In the chronic phase of NTS nephritis at week 20, semiquantitative evaluation of the glomerular lesions disclosed more severe focal segmental glomerulosclerosis (FSGS) in Group 2 compared with Group 1 animals (glomerular injury score: 14 +/- 10 in Group 1 against 73 +/- 17 in Group 2; p < 0.01). Accumulations of lipid and foam cells were invariably seen in the sclerotic foci of Group 2 animals. The results indicate that hypercholesterolemia played an important role in the accelerated development of FSGS in rat NTS nephritis.(ABSTRACT TRUNCATED AT 250 WORDS)

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