脂肪储存细胞在小鼠血吸虫肝纤维化中的作用。

A de A Barbosa Júnior, U Pfeifer, Z A Andrade
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引用次数: 17

摘要

研究了曼氏血吸虫尾蚴感染小鼠肝血吸虫肉芽肿中脂肪储存细胞(FSC)的作用。感染后,24只动物接受sc注射维生素A治疗,总剂量为210,000 IU,每周两次,持续3周。另外两组每组24只动物分别是:a)未感染维生素a治疗的动物和(b)未感染的对照组。各组动物于感染后第5 ~ 10周每隔一周处死一次。用光镜和透射电镜检查肝脏切片。在所有维生素a处理的动物中,FSC显示出突出的细胞质脂肪滴,这使得它们可以在光线和电子显微镜下迅速识别。它们大量存在于骨膜肉芽肿中。在受感染的对照组中,肉芽肿中未发现FSC,可能是因为脂滴在向成纤维细胞表型分化的过程中消失了。FSC也出现在间隔纤维化区。这些数据表明,FSC在小鼠肝脏mansoni卵周围肉芽肿形成过程中的局灶性肝门纤维化中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of fat-storing cells in schistosomal hepatic fibrosis of mice.

The involvement of fat-storing cells (FSC) in hepatic schistosomal granuloma was investigated in mice infected with Schistosoma mansoni cercariae. After infection, 24 animals were treated with s.c. injections of vitamin A in a total dose of 210,000 IU given twice a week for 3 weeks. Two other groups of 24 animals each were: a) non-infected vitamin A-treated animals and (b) untreated infected controls. Animals from all groups were killed at weekly intervals from the 5th through the 10th week following infection. Pieces of liver were examined by light microscopy and transmission electron microscopy. In all vitamin A-treated animals, FSC disclosed prominent cytoplasmic fat droplets, which permitted their prompt identification in the light and in the electron microscope. They were found in large numbers as a constituent of periovular granulomas. In infected controls, FSC were not identified in granulomas, possibly because lipid droplets disappeared during differentiation to the fibroblastic phenotype. FSC also appeared within areas of septal fibrosis. These data suggest that FSC play an important part in focal portohepatic fibrosis during granuloma formation around S. mansoni eggs in the liver of mice.

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