{"title":"皮肤t细胞淋巴瘤患者表皮白细胞介素-8免疫反应性。","authors":"J M Wismer, R C McKenzie, D N Sauder","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The histopathologic hallmark of cutaneous T-cell lymphoma is collections of malignant T cells in the epidermis. The mechanism(s) involved in the T-cell localization is unknown, however recent evidence suggests that cytokines play a role. Since Interleukin-8 functions both as a leukocyte and lymphocyte chemoattractant, we measured IL-8 expression in skin of cutaneous T-cell lymphoma patients. Lesional skin was obtained by biopsy from cutaneous T-cell lymphoma (CTCL) patients and patients suffering from other dermatoses and subjected to in situ hybridization with a 35S-Riboprobe and immunofluorescence to detect IL-8. Fourteen out of 15 lesional biopsies from histologically proven CTCL patients displayed intense epidermal IL-8 immunoreactivity compared to negligible reactivity in sections from 14 normal patients and other inflammatory dermatoses. These results were corroborated by strong hybridization signals using IL-8 probes in only the lesional CTCL skin. Lesional CTCL epidermis contains elevated levels of IL-8 mRNA and protein suggesting a role for this cytokine in the pathogenesis of the disease.</p>","PeriodicalId":77246,"journal":{"name":"Lymphokine and cytokine research","volume":"13 1","pages":"21-7"},"PeriodicalIF":0.0000,"publicationDate":"1994-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Interleukin-8 immunoreactivity in epidermis of cutaneous T-cell lymphoma patients.\",\"authors\":\"J M Wismer, R C McKenzie, D N Sauder\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The histopathologic hallmark of cutaneous T-cell lymphoma is collections of malignant T cells in the epidermis. The mechanism(s) involved in the T-cell localization is unknown, however recent evidence suggests that cytokines play a role. Since Interleukin-8 functions both as a leukocyte and lymphocyte chemoattractant, we measured IL-8 expression in skin of cutaneous T-cell lymphoma patients. Lesional skin was obtained by biopsy from cutaneous T-cell lymphoma (CTCL) patients and patients suffering from other dermatoses and subjected to in situ hybridization with a 35S-Riboprobe and immunofluorescence to detect IL-8. Fourteen out of 15 lesional biopsies from histologically proven CTCL patients displayed intense epidermal IL-8 immunoreactivity compared to negligible reactivity in sections from 14 normal patients and other inflammatory dermatoses. These results were corroborated by strong hybridization signals using IL-8 probes in only the lesional CTCL skin. Lesional CTCL epidermis contains elevated levels of IL-8 mRNA and protein suggesting a role for this cytokine in the pathogenesis of the disease.</p>\",\"PeriodicalId\":77246,\"journal\":{\"name\":\"Lymphokine and cytokine research\",\"volume\":\"13 1\",\"pages\":\"21-7\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lymphokine and cytokine research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lymphokine and cytokine research","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Interleukin-8 immunoreactivity in epidermis of cutaneous T-cell lymphoma patients.
The histopathologic hallmark of cutaneous T-cell lymphoma is collections of malignant T cells in the epidermis. The mechanism(s) involved in the T-cell localization is unknown, however recent evidence suggests that cytokines play a role. Since Interleukin-8 functions both as a leukocyte and lymphocyte chemoattractant, we measured IL-8 expression in skin of cutaneous T-cell lymphoma patients. Lesional skin was obtained by biopsy from cutaneous T-cell lymphoma (CTCL) patients and patients suffering from other dermatoses and subjected to in situ hybridization with a 35S-Riboprobe and immunofluorescence to detect IL-8. Fourteen out of 15 lesional biopsies from histologically proven CTCL patients displayed intense epidermal IL-8 immunoreactivity compared to negligible reactivity in sections from 14 normal patients and other inflammatory dermatoses. These results were corroborated by strong hybridization signals using IL-8 probes in only the lesional CTCL skin. Lesional CTCL epidermis contains elevated levels of IL-8 mRNA and protein suggesting a role for this cytokine in the pathogenesis of the disease.