维生素A缺乏诱导大鼠气管鳞状化生中朗格汉斯细胞的迁移和成熟。

S Hosokawa, M Shinzato, C Kaneko, M Shamoto
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引用次数: 19

摘要

用免疫组织化学和电镜观察了维生素A缺乏对大鼠气管鳞状化生中朗格汉斯细胞(LCs)迁移和成熟的影响。在化生早期即基底细胞增生时,未见有Birbeck颗粒的细胞,但有无桥粒的细胞,具有未成熟细胞的形态特征。它们与巨噬细胞和淋巴细胞等炎症细胞明显不同,因此被认为是lc的前体。在分层阶段,少量Ia-和PKCII阳性细胞被识别。超微结构为卵形核、游离核糖体多、树突少的未成熟细胞。细胞质暗,高尔基区可见少量BGs和非典型颗粒(AGs)。在锥化早期,LCs具有部分拟合核,核仁突出,高尔基复合物发育良好。高尔基地区存在大量的岩体和岩体,并存在着两者之间的过渡性构造。在显示成熟鳞状化生的上皮中,可以看到许多Ia和pkcii阳性的树突状细胞。多数为典型的成熟细胞,核分叶状,细胞质清晰,树突突出。BGs和AGs的数量少于凝化早期的LCs,这些颗粒分布在细胞质中。在最后阶段,基底细胞分化为扁平上皮,可见少量上皮细胞。(摘要删节250字)
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Migration and maturation of Langerhans cells in squamous metaplasia of the rat trachea induced by vitamin A deficiency.

The migration and maturation of Langerhans cells (LCs) in rat tracheal squamous metaplasia due to vitamin A deficiency were investigated immunohistochemically and electron microscopically. In the early stage of metaplasia, i.e. basal cell hyperplasia, no LCs with Birbeck granules (BGs) could be found, but there were desmosome-free cells which had the morphological characteristic of immature LCs. They were clearly different from inflammatory cells such as macrophages and lymphocytes, and were, therefore, considered to be precursors of LCs. In the stage of stratification, small numbers of Ia- and protein kinase C type II (PKCII)-positive cells were recognized. Ultrastructurally they were immature LCs with ovoid nuclei, many free ribosomes and few dendrites. The cytoplasm was dark and a few BGs and atypical granules (AGs) could be seen in the Golgi area. In the early stage of cornification, LCs with partially intended nuclei, prominent nucleoli and well-developed Golgi complexes were found. There were many BGs and AGs and structures transitional between them in the Golgi areas. In epithelium showing mature squamous metaplasia, many Ia- and PKCII-positive dendritic cells could be seen. Most of these were typical mature LCs with lobulated nuclei, clear cytoplasm and prominent dendritic processes. The number of BGs and AGs were fewer than in the LCs found in the early stage of cornification, and these granules were distributed throughout the cytoplasm. In the final stage, where the basal cells had differentiated into a flatter epithelium, few LCs could be seen.(ABSTRACT TRUNCATED AT 250 WORDS)

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