黑腹果蝇种亚群α -淀粉酶酶学特征的比较研究。

Idengaku zasshi Pub Date : 1994-06-01 DOI:10.1266/jjg.69.251
H Shibata, T Yamazaki
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引用次数: 15

摘要

对黑腹果蝇亚群中6个兄弟种α -淀粉酶的酶学特征进行了比较研究。最适pH值分别为:黑腹龙、毛里求斯龙、拟象龙7.6,直立龙、泰西耶利龙、雅库巴龙7.4。所有物种的最适温度均为37℃。然而,有两种类型的温度依赖性;在D. melanogaster, D. mauritiana和D. simulans中观察到的严格的一个,以及在D. erecta, D. teissieri和D. yakuba中观察到的平坦的一个。这些分离与该物种亚群的一般系统发育一致。在所有被研究的物种中,对底物的依赖性几乎相同。并对各物种的Vmax和Km进行了估计。我们发现这两个物种在酶学特征上有明显的差异。因此,这些差异可能反映了该物种亚群中淀粉酶系统对物种形成的适应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A comparative study of the enzymological features of alpha-amylase in the Drosophila melanogaster species subgroup.

A comparative study was done on the enzymological features of alpha-amylase among six sibling species belonging to the Drosophila melanogaster species subgroup. The pH optima were 7.6 for D. melanogaster, D. mauritiana and D. simulans and 7.4 for D. erecta, D. teissieri and D. yakuba. The temperature optima were commonly 37 C in all species studied. However, there are two types of temperature dependence; a strict one as observed in D. melanogaster, D. mauritiana and D. simulans, and a flat one as observed in D. erecta, D. teissieri and D. yakuba. These separations are consistent with the general phylogeny within this species subgroup. The substrate dependence was almost the same in all the species studied. The Vmax and Km were also estimated for each species. We found clear differences in the enzymological features between the species. Thus these differences might reflect an adaptation of the amylase enzyme system to speciation in this species subgroup.

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