{"title":"转化生长因子-β1对大鼠肠上皮细胞系(rie-1)的抑制增殖作用依赖于细胞群密度和培养传代数","authors":"Roger D. Smith","doi":"10.1016/0020-711X(94)90083-3","DOIUrl":null,"url":null,"abstract":"<div><p></p><ul><li><span>1.</span><span><p>1. The proliferation of RIE-1 rat intestinal epithelial cells was potently but reversibly inhibited by transforming growth factor-β<sub>1</sub> (TGF-β<sub>1</sub>). In early-passage cultures, complete growth arrest was observed when sparse cultures were treated with TGF-β<sub>1</sub> (1 ng/ml).</p></span></li><li><span>2.</span><span><p>2. However, increasing the initial cell culture density resulted in decreased TGF-β<sub>1</sub>-mediated inhibition of cell proliferation.</p></span></li><li><span>3.</span><span><p>3. Independent of this population density effect, RIE-1 cells also exhibit a marked phenotypic transition around passage-8 to -10, such that later-passage cells were less responsive to growth inhibition by TGF-β<sub>1</sub>].</p></span></li></ul></div>","PeriodicalId":13733,"journal":{"name":"International Journal of Biochemistry","volume":"26 7","pages":"Pages 899-903"},"PeriodicalIF":0.0000,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711X(94)90083-3","citationCount":"7","resultStr":"{\"title\":\"The anti-proliferative action of transforming growth factor-β1 on a rat intestinal epithelial cell line (rie-1) is dependent on cell population density and culture passage number\",\"authors\":\"Roger D. Smith\",\"doi\":\"10.1016/0020-711X(94)90083-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p></p><ul><li><span>1.</span><span><p>1. The proliferation of RIE-1 rat intestinal epithelial cells was potently but reversibly inhibited by transforming growth factor-β<sub>1</sub> (TGF-β<sub>1</sub>). In early-passage cultures, complete growth arrest was observed when sparse cultures were treated with TGF-β<sub>1</sub> (1 ng/ml).</p></span></li><li><span>2.</span><span><p>2. However, increasing the initial cell culture density resulted in decreased TGF-β<sub>1</sub>-mediated inhibition of cell proliferation.</p></span></li><li><span>3.</span><span><p>3. Independent of this population density effect, RIE-1 cells also exhibit a marked phenotypic transition around passage-8 to -10, such that later-passage cells were less responsive to growth inhibition by TGF-β<sub>1</sub>].</p></span></li></ul></div>\",\"PeriodicalId\":13733,\"journal\":{\"name\":\"International Journal of Biochemistry\",\"volume\":\"26 7\",\"pages\":\"Pages 899-903\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0020-711X(94)90083-3\",\"citationCount\":\"7\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0020711X94900833\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0020711X94900833","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The anti-proliferative action of transforming growth factor-β1 on a rat intestinal epithelial cell line (rie-1) is dependent on cell population density and culture passage number
1.
1. The proliferation of RIE-1 rat intestinal epithelial cells was potently but reversibly inhibited by transforming growth factor-β1 (TGF-β1). In early-passage cultures, complete growth arrest was observed when sparse cultures were treated with TGF-β1 (1 ng/ml).
2.
2. However, increasing the initial cell culture density resulted in decreased TGF-β1-mediated inhibition of cell proliferation.
3.
3. Independent of this population density effect, RIE-1 cells also exhibit a marked phenotypic transition around passage-8 to -10, such that later-passage cells were less responsive to growth inhibition by TGF-β1].
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