Tatsuji Nishihara , Tomihisa Takahashi , Yuichi Ishihara , Hidenobu Senpuku , Naoyuki Takahashi , Tatsuo Suda , Toshihiko Koga
{"title":"膜相关的白细胞介素-1促进体外破骨细胞样细胞的形成","authors":"Tatsuji Nishihara , Tomihisa Takahashi , Yuichi Ishihara , Hidenobu Senpuku , Naoyuki Takahashi , Tatsuo Suda , Toshihiko Koga","doi":"10.1016/S0169-6009(08)80204-8","DOIUrl":null,"url":null,"abstract":"<div><p>The effect of paraformaldehyde-fixed murine macrophage P388D<sub>1</sub> cells on osteoclast-like cell formation was investigated in mouse marrow cultures. When mouse marrow cells were cocultured for 8 days with paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with lipopoly-saccaride (LPS), many tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells were formed. Non-stimulated, and paraformaldehyde-fixed P388D<sub>1</sub> cells did not induce the formation of TRACP-positive multinucleated cells. Salmon calcitonin and indomethacin strongly inhibited the paraformaldehyde-fixed, LPS-stimulated P388D<sub>1</sub> cells-induced TRACP-positive multinucleated cell formation. Monospecific anti-mouse recombinant IL-lα serum inhibited TRACP-positive multinucleated cell formation in the presence of paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with LPS. These results suggest that membrane-associated IL-1 on macrophages is responsible for the resorptive effect of paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with LPS.</p></div>","PeriodicalId":77047,"journal":{"name":"Bone and mineral","volume":"25 1","pages":"Pages 15-24"},"PeriodicalIF":0.0000,"publicationDate":"1994-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0169-6009(08)80204-8","citationCount":"12","resultStr":"{\"title\":\"Membrane-associated interleukin-1 promotes osteoclast-like cell formation in vitro\",\"authors\":\"Tatsuji Nishihara , Tomihisa Takahashi , Yuichi Ishihara , Hidenobu Senpuku , Naoyuki Takahashi , Tatsuo Suda , Toshihiko Koga\",\"doi\":\"10.1016/S0169-6009(08)80204-8\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The effect of paraformaldehyde-fixed murine macrophage P388D<sub>1</sub> cells on osteoclast-like cell formation was investigated in mouse marrow cultures. When mouse marrow cells were cocultured for 8 days with paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with lipopoly-saccaride (LPS), many tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells were formed. Non-stimulated, and paraformaldehyde-fixed P388D<sub>1</sub> cells did not induce the formation of TRACP-positive multinucleated cells. Salmon calcitonin and indomethacin strongly inhibited the paraformaldehyde-fixed, LPS-stimulated P388D<sub>1</sub> cells-induced TRACP-positive multinucleated cell formation. Monospecific anti-mouse recombinant IL-lα serum inhibited TRACP-positive multinucleated cell formation in the presence of paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with LPS. These results suggest that membrane-associated IL-1 on macrophages is responsible for the resorptive effect of paraformaldehyde-fixed P388D<sub>1</sub> cells stimulated with LPS.</p></div>\",\"PeriodicalId\":77047,\"journal\":{\"name\":\"Bone and mineral\",\"volume\":\"25 1\",\"pages\":\"Pages 15-24\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0169-6009(08)80204-8\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bone and mineral\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0169600908802048\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bone and mineral","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0169600908802048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Membrane-associated interleukin-1 promotes osteoclast-like cell formation in vitro
The effect of paraformaldehyde-fixed murine macrophage P388D1 cells on osteoclast-like cell formation was investigated in mouse marrow cultures. When mouse marrow cells were cocultured for 8 days with paraformaldehyde-fixed P388D1 cells stimulated with lipopoly-saccaride (LPS), many tartrate-resistant acid phosphatase (TRACP)-positive multinucleated cells were formed. Non-stimulated, and paraformaldehyde-fixed P388D1 cells did not induce the formation of TRACP-positive multinucleated cells. Salmon calcitonin and indomethacin strongly inhibited the paraformaldehyde-fixed, LPS-stimulated P388D1 cells-induced TRACP-positive multinucleated cell formation. Monospecific anti-mouse recombinant IL-lα serum inhibited TRACP-positive multinucleated cell formation in the presence of paraformaldehyde-fixed P388D1 cells stimulated with LPS. These results suggest that membrane-associated IL-1 on macrophages is responsible for the resorptive effect of paraformaldehyde-fixed P388D1 cells stimulated with LPS.
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