人肾血小板活化因子乙酰水解酶(PAF-AH)

International Journal of Biochemistry Pub Date : 1994-09-01 DOI:10.1016/0020-711X(94)90138-4

Smaragdi Antonopoulou , Constantinos A. Demopoulos , Christos Iatrou , George Moustakas , Panos Zirogiannis

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引用次数: 28

摘要

1.1. PAF-AH在人类肾脏(皮质和髓质)中的活性已被证实，并具有以下特性。不需要Ca2+的存在，似乎与磷脂酶A2.3.3不同。pH最适值在7-7.4.4.4处达到峰值。在- 30°c下稳定4天。主要分布于微粒体部分。皮层和髓质酶的表观Km值分别为0.553和0.207 μM，与血清PAF-AH (1.439 μM)差异显著。髓质和皮质的表观分子量分别为60,000和25,000，与血清PAF-AH(94,000)不同。

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Platelet-activating factor acetylhydrolase (PAF-AH) in human kidney

1.
1. PAF-AH activity in human kidney (cortex and medulla) has been demonstrated and shares the following properties.
2.
2. Does not require the presence of Ca²⁺ and appears to be different from phospholipase A₂.
3.
3. The pH optimum shows a peak at 7–7.4.
4.
4. It is stable for 4 days at −30°C.
5.
5. It is mainly distributed in the microsomal fraction.
6.
6. The apparent K_m, values of the enzymes of cortex and medulla are 0.553 and 0.207 μM, respectively and distinct from serum PAF-AH (1.439 μM).
7.
7. The apparent molecular weight values are 60,000 and 25,000 for medulla and cortex, respectively and distinct from serum PAF-AH (94,000).

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来源期刊

International Journal of Biochemistry

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