G Grassi Zucconi, M Menegazzi, A Carcereri De Prati, S Vescia, G Ranucci, M Bentivoglio
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In basal conditions c-fos expression displayed a highly significant spontaneous oscillation, with the highest level during the darkness hours and the lowest during the light hours. Parallel levels of jun B expression were found in the cortex and striatum, whereas c-jun mRNA remained constantly high throughout 24 h. The periodicity of c-fos and jun B oscillation persisted also when the animals were exposed for 6 days to constant (24h/24h) light or darkness. Such oscillation could instead be inverted by manipulating the rest-activity cycle, i.e. keeping the animals awake during the light hours and allowing them to sleep during the dark hours. We then verified whether the expression of fos and jun could be correlated with states of wakefulness (W) and sleep (S), monitored with EEG recording under behavioral control.(ABSTRACT TRUNCATED AT 250 WORDS)</p>","PeriodicalId":75705,"journal":{"name":"Chronobiologia","volume":"21 1-2","pages":"93-7"},"PeriodicalIF":0.0000,"publicationDate":"1994-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Different programs of gene expression are associated with different phases of the 24h and sleep-wake cycles.\",\"authors\":\"G Grassi Zucconi, M Menegazzi, A Carcereri De Prati, S Vescia, G Ranucci, M Bentivoglio\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The Fos and Jun proteins are encoded by proto-oncogenes acting as immediate early genes in that they are rapidly induced by different kinds of stimuli in the nervous system. 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引用次数: 0
摘要
Fos和Jun蛋白是由原癌基因编码的,它们作为直接早期基因,在神经系统中被不同种类的刺激迅速诱导。这两种蛋白与DNA结合,调节基因转录,从而决定了神经元对应用刺激反应的特异性。我们研究了在没有外源刺激的情况下,这些基因的表达是否在24小时内发生变化。雄性成年Wistar大鼠(200克体重),保持12h/12h明暗循环,从0700开始每4h处死一次。采用Northern blot杂交方法研究了c-fos、c-jun和jun B mrna在6个不同脑区的表达,并采用原位杂交和免疫组织化学方法研究了c-fos的表达。在基本条件下,c-fos表达表现出高度显著的自发振荡,暗时表达量最高,亮时表达量最低。在皮质和纹状体中发现了平行水平的jun B表达,而c-jun mRNA在24小时内持续保持高水平。在连续(24小时/24小时)光照或黑暗条件下暴露6天,c-fos和jun B振荡的周期性也持续存在。相反,这种振荡可以通过控制休息-活动周期来逆转,即在白天让动物保持清醒,在黑夜让它们睡觉。然后,我们验证了fos和jun的表达是否与清醒状态(W)和睡眠状态(S)相关,并在行为控制下用脑电图记录监测。(摘要删节250字)
Different programs of gene expression are associated with different phases of the 24h and sleep-wake cycles.
The Fos and Jun proteins are encoded by proto-oncogenes acting as immediate early genes in that they are rapidly induced by different kinds of stimuli in the nervous system. These two proteins bind to DNA regulating gene transcription, and thus determining the specificity of the neuronal response to the applied stimulation. We investigated whether the expression of these genes undergoes a variation during 24h in the absence of exogenous stimulation. Male Wistar adult (200 gr. body weight) rats, kept under a 12h/12h light-dark cycle, were sacrificed every 4h starting at 0700. The expression of c-fos, c-jun and jun B mRNAs was studied in six different brain areas by means of Northern blot hybridization, c-fos expression was also studied with in situ hybridization and immunohistochemistry. In basal conditions c-fos expression displayed a highly significant spontaneous oscillation, with the highest level during the darkness hours and the lowest during the light hours. Parallel levels of jun B expression were found in the cortex and striatum, whereas c-jun mRNA remained constantly high throughout 24 h. The periodicity of c-fos and jun B oscillation persisted also when the animals were exposed for 6 days to constant (24h/24h) light or darkness. Such oscillation could instead be inverted by manipulating the rest-activity cycle, i.e. keeping the animals awake during the light hours and allowing them to sleep during the dark hours. We then verified whether the expression of fos and jun could be correlated with states of wakefulness (W) and sleep (S), monitored with EEG recording under behavioral control.(ABSTRACT TRUNCATED AT 250 WORDS)