球形芽孢杆菌蚊源菌株胞外丝氨酸蛋白酶的合成。

C Dumusois, F G Priest
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引用次数: 11

摘要

9株对蚊子幼虫有毒性的球形芽孢杆菌在酪蛋白营养琼脂上培养时产生水解晕。在批量培养中,球形芽孢杆菌BSE 18的蛋白酶合成发生在指数生长阶段,并受到高浓度蛋白胨的抑制。该菌胞外蛋白酶在pH约10.2时表现出最佳活性,受苯基甲基磺酰氯和凝乳抑素的抑制,但不受大豆胰蛋白酶抑制剂和EDTA的抑制。n - cbz -甘氨酸-硝基苯基酯的水解与主酶为丝氨酸蛋白酶一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Extracellular serine protease synthesis by mosquito-pathogenic strains of Bacillus sphaericus.

Nine strains of Bacillus sphaericus toxic to mosquito larvae produced haloes of hydrolysis when cultured on casein-nutrient agar. In batch culture, protease synthesis by B. sphaericus BSE 18 occurred during exponential growth and was repressed by high concentrations of peptone. Extracellular protease from this bacterium showed optimal activity at about pH 10.2, was inhibited by phenylmethylsulphonyl chloride and chymostatin but not soybean trypsin inhibitor or EDTA. Hydrolysis of N-CBZ-glycine-nitrophenyl ester was consistent with the major enzyme being a serine protease.

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