小鼠黑色素瘤细胞表面抗原的免疫原性鉴定。

D Johnston, S el Rouby, J C Bystryn
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引用次数: 1

摘要

主动免疫B16黑色素瘤细胞或疫苗可诱导同基因小鼠的抗黑色素瘤免疫反应。刺激对这种肿瘤免疫的免疫原性抗原尚未确定。在本研究中,我们用免疫B16黑色素瘤疫苗诱导的抗黑色素瘤抗体作为探针,检测了几种B16黑色素瘤抗原在同基因小鼠中的免疫原性。这些抗原通过SDS-PAGE和特异性免疫沉淀放射自显影分析鉴定。它们是细胞表面组分,分子量分别为41、46、50、75、80和104 KD。所有这些抗原均可在同基因和异种黑色素瘤以及一些不相关的同基因肿瘤中表达,但不能在正常的同基因细胞、异种黑色素细胞或从新鲜肿瘤中获得的B16黑色素瘤细胞或在特定培养基中生长的B16黑色素瘤细胞中表达。这些抗原不同于B16黑色素瘤细胞表达的小鼠病毒抗原,也不同于用于培养用于疫苗生产的细胞的培养基成分。这些结果表明,几种B16黑色素瘤细胞表面抗原在同基因小鼠中具有免疫原性。这些抗原的表达似乎与恶性转化有关,因为它们在所有研究的黑色素瘤和其他一些癌症中都被发现,但在正常细胞中却没有。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of melanoma cell surface antigens immunogenic in mice.

Active immunization to B16 melanoma cells or vaccines induces anti-melanoma immune responses in syngeneic mice. The immunogenic antigens stimulating immunity to this tumor have not been identified. In this study we detected several B16 melanoma antigens immunogenic in syngeneic mice using as probes antimelanoma antibodies induced by immunization to B16 melanoma vaccines. These antigens were identified by SDS-PAGE and autoradiographic analysis of specific immunoprecipitates. They were cell-surface components with approximate molecular weights of 41, 46, 50, 75, 80, and 104 KD. All these antigens were expressed by syngeneic and xenogeneic melanomas and by some unrelated syngeneic tumors but not by normal syngeneic cells, xenogeneic melanocytes, or by B16 melanoma cells obtained from fresh tumors or grown in defined medium. The antigens were distinct from murine viral antigens expressed by B16 melanoma cells and from components of the culture medium used to grow cells for vaccine production. These results indicate that several B16 melanoma cell-surface antigens are immunogenic in syngeneic mice. Expression of these antigens appears to be related to malignant transformation as they were found on all melanomas studied, and some other cancers, but not on normal cells.

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