{"title":"早期人类卵黄囊的聚糖。","authors":"C J Jones, E Jauniaux, R W Stoddart","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The pattern of glycan distribution in the early human yolk sac has been investigated using a panel of lectins. Two 6-week and one 8-week human yolk sacs, and one 8-week fetal liver from live, ectopic pregnancies were fixed and embedded in epoxy resin. Lectin histochemistry was carried out on sections of these tissues using 23 biotinylated lectins and an avidin-biotin peroxidase revealing system. Mesothelial surfaces expressed most subsets of N-glycans (other than high mannose types), N-acetyl-lactosamine, sialic acid, and alpha 1,6-N-acetylgalactosamine. Endodermal surface and lateral membranes resembled those of mesothelium, but showed a preponderance of alpha 2,6-sialyl residues. Most intracellular granules contained N-glycan. There was a marked heterogeneity of granules in the endodermal cells, with different subsets varying in both staining and positional characteristics. The mesenchymal matrix bound most of the lectins used in the study, and expressed fucosyl residues which were also detected in the endothelium. Fetal liver parenchyma showed very similar staining patterns to those seen in the endoderm except for the distribution of N-acetylglucosamine, which was sparse. Despite some common features, each germ cell layer had a distinct 'glycotype', with some saccharides showing extreme topographical restriction.</p>","PeriodicalId":22439,"journal":{"name":"The Histochemical Journal","volume":"27 3","pages":"210-21"},"PeriodicalIF":0.0000,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Glycans of the early human yolk sac.\",\"authors\":\"C J Jones, E Jauniaux, R W Stoddart\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The pattern of glycan distribution in the early human yolk sac has been investigated using a panel of lectins. Two 6-week and one 8-week human yolk sacs, and one 8-week fetal liver from live, ectopic pregnancies were fixed and embedded in epoxy resin. Lectin histochemistry was carried out on sections of these tissues using 23 biotinylated lectins and an avidin-biotin peroxidase revealing system. Mesothelial surfaces expressed most subsets of N-glycans (other than high mannose types), N-acetyl-lactosamine, sialic acid, and alpha 1,6-N-acetylgalactosamine. Endodermal surface and lateral membranes resembled those of mesothelium, but showed a preponderance of alpha 2,6-sialyl residues. Most intracellular granules contained N-glycan. There was a marked heterogeneity of granules in the endodermal cells, with different subsets varying in both staining and positional characteristics. The mesenchymal matrix bound most of the lectins used in the study, and expressed fucosyl residues which were also detected in the endothelium. Fetal liver parenchyma showed very similar staining patterns to those seen in the endoderm except for the distribution of N-acetylglucosamine, which was sparse. Despite some common features, each germ cell layer had a distinct 'glycotype', with some saccharides showing extreme topographical restriction.</p>\",\"PeriodicalId\":22439,\"journal\":{\"name\":\"The Histochemical Journal\",\"volume\":\"27 3\",\"pages\":\"210-21\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Histochemical Journal\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Histochemical Journal","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
用一组凝集素研究了早期人类卵黄囊中聚糖分布的模式。将2个6周和1个8周的卵黄囊和1个8周的异位活胎肝固定并包埋在环氧树脂中。使用23种生物素化凝集素和亲和素-生物素过氧化物酶揭示系统对这些组织切片进行凝集素组织化学。间皮表面表达大多数n -聚糖亚群(高甘露糖类型除外)、n -乙酰-乳胺、唾液酸和α 1,6- n -乙酰半乳糖胺。内胚层表面和外侧膜与间皮相似,但α 2,6-唾液残基占优势。大多数细胞内颗粒含有n -聚糖。内胚层细胞颗粒具有明显的异质性,不同亚群在染色和位置特征上都有所不同。间质基质结合了研究中使用的大部分凝集素,并表达了在内皮中也检测到的聚焦基残基。胎儿肝实质的染色模式与内胚层非常相似,只是n -乙酰氨基葡萄糖分布稀疏。尽管有一些共同的特征,每个生殖细胞层都有一个独特的“糖型”,其中一些糖表现出极端的地形限制。
The pattern of glycan distribution in the early human yolk sac has been investigated using a panel of lectins. Two 6-week and one 8-week human yolk sacs, and one 8-week fetal liver from live, ectopic pregnancies were fixed and embedded in epoxy resin. Lectin histochemistry was carried out on sections of these tissues using 23 biotinylated lectins and an avidin-biotin peroxidase revealing system. Mesothelial surfaces expressed most subsets of N-glycans (other than high mannose types), N-acetyl-lactosamine, sialic acid, and alpha 1,6-N-acetylgalactosamine. Endodermal surface and lateral membranes resembled those of mesothelium, but showed a preponderance of alpha 2,6-sialyl residues. Most intracellular granules contained N-glycan. There was a marked heterogeneity of granules in the endodermal cells, with different subsets varying in both staining and positional characteristics. The mesenchymal matrix bound most of the lectins used in the study, and expressed fucosyl residues which were also detected in the endothelium. Fetal liver parenchyma showed very similar staining patterns to those seen in the endoderm except for the distribution of N-acetylglucosamine, which was sparse. Despite some common features, each germ cell layer had a distinct 'glycotype', with some saccharides showing extreme topographical restriction.