{"title":"抗原刺激全血中干扰素产生的昼夜节律性。","authors":"N Petrovsky, P McNair, L C Harrison","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-gamma. With this assay we have found a large circadian variation in tetanus- (acrophase 00(00) p < 0.001) and PPD- (acrophase 00(08) p < 0.001) stimulated IFN-gamma production. IFN-gamma production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-gamma production may be secondary to circadian variation in plasma cortisol levels (acrophase 11(06)). The demonstration of circadian rhythmicity in antigen-stimulated IFN-gamma production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.</p>","PeriodicalId":75705,"journal":{"name":"Chronobiologia","volume":"21 3-4","pages":"293-300"},"PeriodicalIF":0.0000,"publicationDate":"1994-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Circadian rhythmicity of interferon-gamma production in antigen-stimulated whole blood.\",\"authors\":\"N Petrovsky, P McNair, L C Harrison\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-gamma. With this assay we have found a large circadian variation in tetanus- (acrophase 00(00) p < 0.001) and PPD- (acrophase 00(08) p < 0.001) stimulated IFN-gamma production. IFN-gamma production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-gamma production may be secondary to circadian variation in plasma cortisol levels (acrophase 11(06)). The demonstration of circadian rhythmicity in antigen-stimulated IFN-gamma production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.</p>\",\"PeriodicalId\":75705,\"journal\":{\"name\":\"Chronobiologia\",\"volume\":\"21 3-4\",\"pages\":\"293-300\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Chronobiologia\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chronobiologia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Circadian rhythmicity of interferon-gamma production in antigen-stimulated whole blood.
Recently, assessment of T cell function has been refined by the ability to measure cytokines produced by activated T cells. We developed a whole blood assay to detect antigen-activated T cells that produce IFN-gamma. With this assay we have found a large circadian variation in tetanus- (acrophase 00(00) p < 0.001) and PPD- (acrophase 00(08) p < 0.001) stimulated IFN-gamma production. IFN-gamma production is inversely correlated with plasma cortisol (r = -0.5), suggesting that variation in IFN-gamma production may be secondary to circadian variation in plasma cortisol levels (acrophase 11(06)). The demonstration of circadian rhythmicity in antigen-stimulated IFN-gamma production is relevant to the diagnostic use of whole blood assays and, in addition, may have implications for the therapy of immuno-inflammatory diseases.
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