胰岛素依赖型糖尿病儿童和非糖尿病儿童的脂蛋白(a)及其他危险因素

Diabete & metabolisme Pub Date : 1994-11-01
M T Martinez, O Ramos, N Carretero, M Calvillan, M D Gutierrez-Lopez, P Cuesta, M Serrano-Rios
{"title":"胰岛素依赖型糖尿病儿童和非糖尿病儿童的脂蛋白(a)及其他危险因素","authors":"M T Martinez,&nbsp;O Ramos,&nbsp;N Carretero,&nbsp;M Calvillan,&nbsp;M D Gutierrez-Lopez,&nbsp;P Cuesta,&nbsp;M Serrano-Rios","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Aims: </strong>To study serum Lp(a) levels and other metabolic cardiovascular risk factors in children with Type 1 diabetes mellitus (DM) compared to sex and age matched nondiabetic children. The correlation of Lp(a) serum levels and other lipid parameters with HbA1c concentrations in diabetic children was investigated.</p><p><strong>Design: </strong>Transversal observational study.</p><p><strong>Target population: </strong>36 C-peptide negative Type 1 DM children without microalbuminuria and no macromicrovascular or neurological complications, aged 8 to 15 years; 17 boys, 19 girls. Mean duration of Type 1 DM was 4.99 +/- 3.04 years, daily insulin need were 32.79 +/- 12.64 Units. 41 healthy children with no family history of DM, aged from 8 to 15 years, 26 boys, 15 girls, were studied in parallel as the control group.</p><p><strong>Methods: </strong>Serum total cholesterol (TC) and triglycerides (TG) were assayed by enzymatic methods, high-density lipoprotein (HDL) cholesterol by enzymatic method after precipitation of very-low-density (VLDL) and low-density lipoprotein (LDL) fractions. The LDL fractions was estimated after serum precipitation as the difference between total cholesterol and supernatant cholesterol concentrations. Apo-AI, apo-AII and apo-B were measured by radial immunodiffusion assays. Serum Lp(a) was measured by monoclonal anti-Lp(a) antibody (ELISA) method and whole blood glycosylated hemoglobin A1c (HbA1c) by high resolution liquid chromatography.</p><p><strong>Results: </strong>HbA1c concentration in diabetic children was 7.51 +/- 54% vs 4.16 +/- 0.35% in non diabetic children. Lp(a) serum levels did not significantly differ among both groups (25 +/- 22 mg/dl in diabetics subjects, 22 +/- 22 mg/dl in controls). Significant correlation was found between HbA1c levels and each of TC, LDL and TG serum concentrations in the diabetic group. Lp(a) levels were correlated with glycated hemoglobin in the whole diabetic group. But, in the 2 patients with the poorest metabolic control (HbA1c 10.5%) were excluded, the correlation disappeared.</p><p><strong>Conclusions: </strong>In 36 children aged 5-15 years with uncomplicated Type 1 DM lasting less than 15 years, Lp(a) serum levels did not differ from age-matched controls but highest Lp(a) values were associated with poorest metabolic control.</p>","PeriodicalId":11111,"journal":{"name":"Diabete & metabolisme","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1994-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Lipoprotein (a) and other risk factors in children with insulin-dependent diabetes mellitus and children without diabetes.\",\"authors\":\"M T Martinez,&nbsp;O Ramos,&nbsp;N Carretero,&nbsp;M Calvillan,&nbsp;M D Gutierrez-Lopez,&nbsp;P Cuesta,&nbsp;M Serrano-Rios\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aims: </strong>To study serum Lp(a) levels and other metabolic cardiovascular risk factors in children with Type 1 diabetes mellitus (DM) compared to sex and age matched nondiabetic children. The correlation of Lp(a) serum levels and other lipid parameters with HbA1c concentrations in diabetic children was investigated.</p><p><strong>Design: </strong>Transversal observational study.</p><p><strong>Target population: </strong>36 C-peptide negative Type 1 DM children without microalbuminuria and no macromicrovascular or neurological complications, aged 8 to 15 years; 17 boys, 19 girls. Mean duration of Type 1 DM was 4.99 +/- 3.04 years, daily insulin need were 32.79 +/- 12.64 Units. 41 healthy children with no family history of DM, aged from 8 to 15 years, 26 boys, 15 girls, were studied in parallel as the control group.</p><p><strong>Methods: </strong>Serum total cholesterol (TC) and triglycerides (TG) were assayed by enzymatic methods, high-density lipoprotein (HDL) cholesterol by enzymatic method after precipitation of very-low-density (VLDL) and low-density lipoprotein (LDL) fractions. The LDL fractions was estimated after serum precipitation as the difference between total cholesterol and supernatant cholesterol concentrations. Apo-AI, apo-AII and apo-B were measured by radial immunodiffusion assays. Serum Lp(a) was measured by monoclonal anti-Lp(a) antibody (ELISA) method and whole blood glycosylated hemoglobin A1c (HbA1c) by high resolution liquid chromatography.</p><p><strong>Results: </strong>HbA1c concentration in diabetic children was 7.51 +/- 54% vs 4.16 +/- 0.35% in non diabetic children. Lp(a) serum levels did not significantly differ among both groups (25 +/- 22 mg/dl in diabetics subjects, 22 +/- 22 mg/dl in controls). Significant correlation was found between HbA1c levels and each of TC, LDL and TG serum concentrations in the diabetic group. Lp(a) levels were correlated with glycated hemoglobin in the whole diabetic group. But, in the 2 patients with the poorest metabolic control (HbA1c 10.5%) were excluded, the correlation disappeared.</p><p><strong>Conclusions: </strong>In 36 children aged 5-15 years with uncomplicated Type 1 DM lasting less than 15 years, Lp(a) serum levels did not differ from age-matched controls but highest Lp(a) values were associated with poorest metabolic control.</p>\",\"PeriodicalId\":11111,\"journal\":{\"name\":\"Diabete & metabolisme\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diabete & metabolisme\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diabete & metabolisme","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

目的:研究1型糖尿病(DM)儿童与性别和年龄匹配的非糖尿病儿童的血清Lp(a)水平和其他代谢心血管危险因素。探讨糖尿病患儿血清Lp(a)水平及其他脂质参数与HbA1c浓度的相关性。设计:横向观察性研究。目标人群:36例c肽阴性1型糖尿病儿童,无微量白蛋白尿,无大微血管或神经系统并发症,年龄8 - 15岁;17个男孩,19个女孩。1型糖尿病的平均病程为4.99 +/- 3.04年,每日胰岛素需用量为32.79 +/- 12.64单位。41名8 ~ 15岁无糖尿病家族史的健康儿童作为对照组,其中男孩26名,女孩15名。方法:酶促法测定血清总胆固醇(TC)和甘油三酯(TG),极低密度(VLDL)和低密度脂蛋白(LDL)沉淀后酶促法测定高密度脂蛋白(HDL)胆固醇。血清沉淀后LDL分数作为总胆固醇和上清胆固醇浓度的差值。采用径向免疫扩散法检测Apo-AI、apo-AII和apo-B。采用单克隆抗Lp(a)抗体(ELISA)法检测血清Lp(a),采用高分辨液相色谱法检测全血糖化血红蛋白(HbA1c)。结果:糖尿病患儿的HbA1c浓度为7.51 +/- 54%,非糖尿病患儿为4.16 +/- 0.35%。两组血清Lp(a)水平无显著差异(糖尿病患者为25 +/- 22 mg/dl,对照组为22 +/- 22 mg/dl)。糖尿病组患者HbA1c水平与TC、LDL、TG血清浓度均有显著相关性。在整个糖尿病组中,Lp(a)水平与糖化血红蛋白相关。但在排除代谢控制最差(HbA1c 10.5%)的2例患者中,相关性消失。结论:在36例5-15岁无并发症1型糖尿病持续时间少于15年的儿童中,血清Lp(a)水平与年龄匹配的对照组没有差异,但最高的Lp(a)值与最差的代谢控制相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Lipoprotein (a) and other risk factors in children with insulin-dependent diabetes mellitus and children without diabetes.

Aims: To study serum Lp(a) levels and other metabolic cardiovascular risk factors in children with Type 1 diabetes mellitus (DM) compared to sex and age matched nondiabetic children. The correlation of Lp(a) serum levels and other lipid parameters with HbA1c concentrations in diabetic children was investigated.

Design: Transversal observational study.

Target population: 36 C-peptide negative Type 1 DM children without microalbuminuria and no macromicrovascular or neurological complications, aged 8 to 15 years; 17 boys, 19 girls. Mean duration of Type 1 DM was 4.99 +/- 3.04 years, daily insulin need were 32.79 +/- 12.64 Units. 41 healthy children with no family history of DM, aged from 8 to 15 years, 26 boys, 15 girls, were studied in parallel as the control group.

Methods: Serum total cholesterol (TC) and triglycerides (TG) were assayed by enzymatic methods, high-density lipoprotein (HDL) cholesterol by enzymatic method after precipitation of very-low-density (VLDL) and low-density lipoprotein (LDL) fractions. The LDL fractions was estimated after serum precipitation as the difference between total cholesterol and supernatant cholesterol concentrations. Apo-AI, apo-AII and apo-B were measured by radial immunodiffusion assays. Serum Lp(a) was measured by monoclonal anti-Lp(a) antibody (ELISA) method and whole blood glycosylated hemoglobin A1c (HbA1c) by high resolution liquid chromatography.

Results: HbA1c concentration in diabetic children was 7.51 +/- 54% vs 4.16 +/- 0.35% in non diabetic children. Lp(a) serum levels did not significantly differ among both groups (25 +/- 22 mg/dl in diabetics subjects, 22 +/- 22 mg/dl in controls). Significant correlation was found between HbA1c levels and each of TC, LDL and TG serum concentrations in the diabetic group. Lp(a) levels were correlated with glycated hemoglobin in the whole diabetic group. But, in the 2 patients with the poorest metabolic control (HbA1c 10.5%) were excluded, the correlation disappeared.

Conclusions: In 36 children aged 5-15 years with uncomplicated Type 1 DM lasting less than 15 years, Lp(a) serum levels did not differ from age-matched controls but highest Lp(a) values were associated with poorest metabolic control.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信