DAPI是一种简单灵敏的替代溴化乙啶在琼脂糖凝胶中染色DNA的方法。

Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society Pub Date : 1993-01-01

E Buel, M Schwartz

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引用次数: 0

摘要

我们描述了一种简单的程序，用4'，6-二氨基-2-苯基吲哚(DAPI)染色水平琼脂糖凝胶中的DNA。该方法具有溴化乙锭的快速和简单性，同时可将染料定位在样品中，限制了偶然的染料污染。

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DAPI, a simple sensitive alternative to ethidium bromide staining of DNA in agarose gels.

We describe a simple procedure to stain DNA in horizontal agarose gels using 4',6-diamidino-2-phenylindole (DAPI). The method has the speed and simplicity of ethidium bromide while localizing the dye in the sample, limiting incidental dye contamination.

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Applied and theoretical electrophoresis : the official journal of the International Electrophoresis Society

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