EGF增强转移性大鼠乳腺腺癌细胞克隆MTLn3对纤维连接蛋白和胶原的附着。

Invasion & metastasis Pub Date : 1995-01-01
B Rohde-Schulz, R B Lichtner
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引用次数: 0

摘要

研究了高转移性大鼠乳腺腺癌MTLn3细胞与基质蛋白的附着及EGF对其的调节作用。在塑料板上涂上不同数量的胶原蛋白或纤维连接蛋白。MTLn3细胞对这两种基质蛋白的粘附均表现出剂量依赖性,然而,它们对胶原蛋白的粘附比对纤维连接蛋白的粘附更有效。当以0.3 ~ 10 ng/ml添加EGF或TGF α 30 min时,观察到对两种基质蛋白的粘附均呈剂量依赖性增加。两种生长因子均为10 ng/ml时,最大刺激达到2倍。然而,EGF在较低浓度(0.3-3 ng/ml)下比TGF α更有效。生长因子刺激粘附的能力也取决于细胞暴露于基质的量。虽然EGF类似地增加了MTLn3细胞与这两种基质蛋白的快速附着,但在胶原上镀的细胞中,随后的细胞扩散和板层延伸的形成速度更快。这些结果提示了EGF受体与特异性整合素活性之间的功能联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
EGF enhances attachment of metastatic rat mammary adenocarcinoma cell clone MTLn3 to fibronectin and collagen.

Attachment of highly metastatic rat mammary adenocarcinoma MTLn3 cells to matrix proteins and its modulation by EGF was examined. Plastic plates were coated with varying amounts of collagen or fibronectin. MTLn3 cells exhibited a dose-dependent adhesion to both matrix proteins, however, they attached more efficiently to collagen than to fibronectin. When EGF or TGF alpha were added at 0.3 to 10 ng/ml for 30 min, a dose-dependent increase in adhesion to both matrix proteins was observed. Maximal stimulation (2-fold) was seen with 10 ng/ml of either growth factor. However, EGF was more potent at lower concentrations (0.3-3 ng/ml) than TGF alpha. The ability of growth factors to stimulate adhesion was also dependent on the amount of matrix the cells were exposed to. While EGF increased rapid attachment of MTLn3 cells to both matrix proteins similarly, subsequent cell spreading and formation of lamellar extensions was faster in cells plated on collagen. These results are suggestive of a functional link between EGF receptor and specific integrin activities.

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