{"title":"白念珠菌肌球蛋白免疫类似物的检测","authors":"Machhour Ghazali, Marie-Helene Rodier, Brahim el Moudni, Nathalie Quellard, Jean-Louis Jacquemin","doi":"10.1006/emyc.1995.1012","DOIUrl":null,"url":null,"abstract":"<div><p>Ghazali, M., Rodier, M.-H., el Moudni, B., Quellard, N., Jacquemin, J.-L. 1995. Detection of myosin immunoanalogue in the yeast <em>Candida albicans. Experimental Mycology</em> 19: 101-110. Detection and localization of myosin immunoanalogue protein in the yeast <em>Candida albicans</em> were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an <em>M<sub>r</sub></em> about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 2","pages":"Pages 101-110"},"PeriodicalIF":0.0000,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1012","citationCount":"4","resultStr":"{\"title\":\"Detection of Myosin Immunoanalogue in the Yeast Candida albicans\",\"authors\":\"Machhour Ghazali, Marie-Helene Rodier, Brahim el Moudni, Nathalie Quellard, Jean-Louis Jacquemin\",\"doi\":\"10.1006/emyc.1995.1012\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Ghazali, M., Rodier, M.-H., el Moudni, B., Quellard, N., Jacquemin, J.-L. 1995. Detection of myosin immunoanalogue in the yeast <em>Candida albicans. Experimental Mycology</em> 19: 101-110. Detection and localization of myosin immunoanalogue protein in the yeast <em>Candida albicans</em> were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an <em>M<sub>r</sub></em> about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.</p></div>\",\"PeriodicalId\":12110,\"journal\":{\"name\":\"Experimental Mycology\",\"volume\":\"19 2\",\"pages\":\"Pages 101-110\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/emyc.1995.1012\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147597585710122\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710122","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 4
摘要
Ghazali, M.; Rodier, M.- h .;el Moudni, B.， Quellard, N.， Jacquemin, J.-L.。1995. 白念珠菌肌球蛋白免疫类似物的检测。实验真菌学19:101-110。采用免疫印迹法、间接免疫荧光法和免疫电镜法对白念珠菌中肌球蛋白免疫类似物蛋白进行了检测和定位。从细胞质提取物和相应膜颗粒的不溶性部分中提取Mr约为110,000的多肽，与针对脊椎动物肌球蛋白的多克隆和单克隆抗体反应。通过免疫荧光和免疫电镜检测，该蛋白位于细胞皮层沿质膜、细胞质和酵母母细胞与芽之间的芽瘢痕区相对应的隔膜中。
Detection of Myosin Immunoanalogue in the Yeast Candida albicans
Ghazali, M., Rodier, M.-H., el Moudni, B., Quellard, N., Jacquemin, J.-L. 1995. Detection of myosin immunoanalogue in the yeast Candida albicans. Experimental Mycology 19: 101-110. Detection and localization of myosin immunoanalogue protein in the yeast Candida albicans were achieved by immunoblotting, indirect immunofluorescence assay, and immunoelectron microscopy. A polypeptide with an Mr about 110,000, from cytosolic extract and insoluble fraction in the corresponding membrane pellet, was reacted with polyclonal and monoclonal antibodies raised against vertebrate muscle myosin. This protein was located by immunofluorescence and immunoelectron microscopy in the cell cortex along the plasmalemma, in the cytoplasm, and in the septum corresponding to bud scar region situated between the yeast-mother cell and the bud.
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