B Quaresima, M T Tiano, A Porcellini, P D'Agostino, M C Faniello, M A Bevilacqua, F Cimino, F Costanzo
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PCR analysis of the H ferritin multigene family reveals the existence of two classes of processed pseudogenes.
The human gene coding for the apoferritin H subunit belongs to a complex multigene family constituted by the expressed gene and by an undefined number of pseudogenes. We have used a strategy based on PCR to amplify specifically the H pseudogenes from a sample of human genomic DNA. With this approach, three new H pseudogenes have been cloned and characterized by DNA sequence analysis. In addition, we have identified a new type of pseudogene, the size of which (700 bp) is caused by multiple detection events in the putative coding region.
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