{"title":"定量分析特定的mRNA转录物使用竞争性PCR分析与电化学发光检测。","authors":"J A Heroux, A M Szczepanik","doi":"10.1101/gr.4.6.327","DOIUrl":null,"url":null,"abstract":"<p><p>Reverse transcriptase-polymerase chain reaction (RT--PCR) has been widely utilized for both the qualitative and quantitative assessment of the levels of specific mRNA transcripts in living systems. Quantitation of specific transcripts has often proved to be problematic because of the difficulty associated with relating the PCR-amplified product to the starting cDNA representing the mRNA of interest. We have overcome these difficulties and have developed a competitive PCR assay employing the property of electrochemiluminescence for the detection of PCR products. This assay possesses the dual advantage of being both nonradioactive and highly sensitive.</p>","PeriodicalId":77315,"journal":{"name":"PCR methods and applications","volume":"4 6","pages":"327-30"},"PeriodicalIF":0.0000,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":"{\"title\":\"Quantitative analysis of specific mRNA transcripts using a competitive PCR assay with electrochemiluminescent detection.\",\"authors\":\"J A Heroux, A M Szczepanik\",\"doi\":\"10.1101/gr.4.6.327\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Reverse transcriptase-polymerase chain reaction (RT--PCR) has been widely utilized for both the qualitative and quantitative assessment of the levels of specific mRNA transcripts in living systems. Quantitation of specific transcripts has often proved to be problematic because of the difficulty associated with relating the PCR-amplified product to the starting cDNA representing the mRNA of interest. We have overcome these difficulties and have developed a competitive PCR assay employing the property of electrochemiluminescence for the detection of PCR products. This assay possesses the dual advantage of being both nonradioactive and highly sensitive.</p>\",\"PeriodicalId\":77315,\"journal\":{\"name\":\"PCR methods and applications\",\"volume\":\"4 6\",\"pages\":\"327-30\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PCR methods and applications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1101/gr.4.6.327\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PCR methods and applications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/gr.4.6.327","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Quantitative analysis of specific mRNA transcripts using a competitive PCR assay with electrochemiluminescent detection.
Reverse transcriptase-polymerase chain reaction (RT--PCR) has been widely utilized for both the qualitative and quantitative assessment of the levels of specific mRNA transcripts in living systems. Quantitation of specific transcripts has often proved to be problematic because of the difficulty associated with relating the PCR-amplified product to the starting cDNA representing the mRNA of interest. We have overcome these difficulties and have developed a competitive PCR assay employing the property of electrochemiluminescence for the detection of PCR products. This assay possesses the dual advantage of being both nonradioactive and highly sensitive.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
