{"title":"北虾壳纯化蛋白的氨基酸序列测定","authors":"S.L. Jacobsen , S.O. Andersen , P. Højrup","doi":"10.1016/0305-0491(94)90004-3","DOIUrl":null,"url":null,"abstract":"<div><p>One of the urea-extractable proteins in the shell of the shrimp <em>Pandalus borealis</em> has been purified, and the complete amino acid sequence has been determined by the combined use of mass spectrometry and Edman degradation of the intact protein as well as of enzymatically derived peptides.</p><p>Characteristic features of the sequence are discussed and compared to sequences from insect cuticular proteins and other structural proteins.</p></div>","PeriodicalId":100294,"journal":{"name":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","volume":"109 2","pages":"Pages 209-217"},"PeriodicalIF":0.0000,"publicationDate":"1994-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0305-0491(94)90004-3","citationCount":"12","resultStr":"{\"title\":\"Amino acid sequence determination of a protein purified from the shell of the shrimp, Pandalus borealis\",\"authors\":\"S.L. Jacobsen , S.O. Andersen , P. Højrup\",\"doi\":\"10.1016/0305-0491(94)90004-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>One of the urea-extractable proteins in the shell of the shrimp <em>Pandalus borealis</em> has been purified, and the complete amino acid sequence has been determined by the combined use of mass spectrometry and Edman degradation of the intact protein as well as of enzymatically derived peptides.</p><p>Characteristic features of the sequence are discussed and compared to sequences from insect cuticular proteins and other structural proteins.</p></div>\",\"PeriodicalId\":100294,\"journal\":{\"name\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"volume\":\"109 2\",\"pages\":\"Pages 209-217\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1994-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/0305-0491(94)90004-3\",\"citationCount\":\"12\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/0305049194900043\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology Part B: Comparative Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0305049194900043","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Amino acid sequence determination of a protein purified from the shell of the shrimp, Pandalus borealis
One of the urea-extractable proteins in the shell of the shrimp Pandalus borealis has been purified, and the complete amino acid sequence has been determined by the combined use of mass spectrometry and Edman degradation of the intact protein as well as of enzymatically derived peptides.
Characteristic features of the sequence are discussed and compared to sequences from insect cuticular proteins and other structural proteins.
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