Christopher Molloy, Maxwell G. Shepherd, Patrick A. Sullivan
{"title":"白色念珠菌包膜n -乙酰氨基葡萄糖酶和海藻糖酶的差异提取","authors":"Christopher Molloy, Maxwell G. Shepherd, Patrick A. Sullivan","doi":"10.1006/emyc.1995.1022","DOIUrl":null,"url":null,"abstract":"<div><p>Molloy, C., Shepherd, M. G., and Sullivan, P. A. 1995. Differential extraction of <em>N</em>-acetylglucosaminidase and trehalase from the cell envelope of <em>Candida albicans. Experimental Mycology</em> 19, 178-185. Dithiothreitol (DTT) extraction of <em>N</em>-acetylglucosaminidase and trehalase from intact <em>Candida albicans</em> ATCC 10261 cells was monitored as an index of cell envelope porosity during <em>N</em>-acetylglucosamine-induced morphogenesis. Trehalase, which is secreted into the cell envelope during starvation and bud-formation, displayed similar extraction kinetics in starved, germ tube-forming, and bud-forming cells, indicating that the mother cell wall remains largely unchanged during morphogenic outgrowth and that the porosity of bud and mother cell walls is similar. <em>N</em>-acetylglucosaminidase, which is secreted specifically during morphogenesis, was released eightfold more rapidly from germ tube-forming than bud-forming cells, reflecting major differences in porosity between bud and germ tube. In addition, by assaying DTT extracts and extracted cell residues, it was found that the total extracellular <em>N</em> -acetylglucosaminidase activity increased 2- to 2.5-fold during DTT treatment. Thus, DTT unmasks a cryptic form of <em>N</em>-acetylglucosaminidase. The cryptic activity was associated with the cell wall fraction.</p></div>","PeriodicalId":12110,"journal":{"name":"Experimental Mycology","volume":"19 3","pages":"Pages 178-185"},"PeriodicalIF":0.0000,"publicationDate":"1995-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1006/emyc.1995.1022","citationCount":"11","resultStr":"{\"title\":\"Differential Extraction of N-Acetylglucosaminidase and Trehalase from the Cell Envelope of Candida albicans\",\"authors\":\"Christopher Molloy, Maxwell G. Shepherd, Patrick A. Sullivan\",\"doi\":\"10.1006/emyc.1995.1022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Molloy, C., Shepherd, M. G., and Sullivan, P. A. 1995. Differential extraction of <em>N</em>-acetylglucosaminidase and trehalase from the cell envelope of <em>Candida albicans. Experimental Mycology</em> 19, 178-185. Dithiothreitol (DTT) extraction of <em>N</em>-acetylglucosaminidase and trehalase from intact <em>Candida albicans</em> ATCC 10261 cells was monitored as an index of cell envelope porosity during <em>N</em>-acetylglucosamine-induced morphogenesis. Trehalase, which is secreted into the cell envelope during starvation and bud-formation, displayed similar extraction kinetics in starved, germ tube-forming, and bud-forming cells, indicating that the mother cell wall remains largely unchanged during morphogenic outgrowth and that the porosity of bud and mother cell walls is similar. <em>N</em>-acetylglucosaminidase, which is secreted specifically during morphogenesis, was released eightfold more rapidly from germ tube-forming than bud-forming cells, reflecting major differences in porosity between bud and germ tube. In addition, by assaying DTT extracts and extracted cell residues, it was found that the total extracellular <em>N</em> -acetylglucosaminidase activity increased 2- to 2.5-fold during DTT treatment. Thus, DTT unmasks a cryptic form of <em>N</em>-acetylglucosaminidase. The cryptic activity was associated with the cell wall fraction.</p></div>\",\"PeriodicalId\":12110,\"journal\":{\"name\":\"Experimental Mycology\",\"volume\":\"19 3\",\"pages\":\"Pages 178-185\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1995-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1006/emyc.1995.1022\",\"citationCount\":\"11\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Experimental Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0147597585710225\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental Mycology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0147597585710225","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Differential Extraction of N-Acetylglucosaminidase and Trehalase from the Cell Envelope of Candida albicans
Molloy, C., Shepherd, M. G., and Sullivan, P. A. 1995. Differential extraction of N-acetylglucosaminidase and trehalase from the cell envelope of Candida albicans. Experimental Mycology 19, 178-185. Dithiothreitol (DTT) extraction of N-acetylglucosaminidase and trehalase from intact Candida albicans ATCC 10261 cells was monitored as an index of cell envelope porosity during N-acetylglucosamine-induced morphogenesis. Trehalase, which is secreted into the cell envelope during starvation and bud-formation, displayed similar extraction kinetics in starved, germ tube-forming, and bud-forming cells, indicating that the mother cell wall remains largely unchanged during morphogenic outgrowth and that the porosity of bud and mother cell walls is similar. N-acetylglucosaminidase, which is secreted specifically during morphogenesis, was released eightfold more rapidly from germ tube-forming than bud-forming cells, reflecting major differences in porosity between bud and germ tube. In addition, by assaying DTT extracts and extracted cell residues, it was found that the total extracellular N -acetylglucosaminidase activity increased 2- to 2.5-fold during DTT treatment. Thus, DTT unmasks a cryptic form of N-acetylglucosaminidase. The cryptic activity was associated with the cell wall fraction.