绵羊小胶质细胞凝集素染色。

Histochemistry Pub Date : 1994-12-01 DOI:10.1007/BF00269580

N A Pennell, S D Hurley, W J Streit

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引用次数: 16

摘要

已知来自Griffonia simplicifolia的B4-isolectin可染色多种物种的小胶质细胞。本报告描述了一种凝集素染色方法，该方法已被修改，以方便在正常羊脑的振动体切片中染色静息小胶质细胞和血管周围细胞。这种改进的方法将组织固定在甲醛或多聚甲醛中，需要在染色前用Triton X-100孵育切片。

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Lectin staining of sheep microglia.

The B4-isolectin from Griffonia simplicifolia is known to stain microglial cells in a variety of species. The present report describes a lectin staining method that has been modified to facilitate staining of resting microglia, as well as perivascular cells, in vibratome sections of normal sheep brain. This modified method employs tissue fixed in formaldehyde or paraformaldehyde and requires incubating sections with Triton X-100 prior to staining.

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Histochemistry

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