自身免疫与正常人血浆及EBV转化淋巴细胞抗tnf α自身抗体的比较。

Human antibodies and hybridomas Pub Date : 1995-01-01
L Tsuchiyama, T Wong, J Kieran, P Boyle, D Penza, G D Wetzel
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引用次数: 0

摘要

为了检测正常个体和自身免疫个体产生循环抗tnf α抗体的能力,我们对10例RA患者、10例SLE患者和5例正常人的血浆样本进行了抗tnf α抗体检测。虽然每个个体测试显示循环IgM抗tnf α抗体,IgG抗tnf α自身抗体主要见于自身免疫性患者。5个正常个体中只有1个,20个自身免疫个体中有15个有血浆IgG抗tnf α抗体。为了检测正常和自身免疫个体从其循环淋巴细胞中产生抗tnf α自身抗体的能力,进行了EBV转化。从13例患者中成功建立了寡克隆永生细胞株,每个细胞株均可分泌可检测的IgM抗tnf α自身抗体。5个正常个体中只有1个转化细胞分泌IgM抗tnf α自身抗体。这些结果表明,尽管正常个体也能够产生这些自身抗体,但自身免疫个体中抗tnf α自身抗体的产生率较高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of anti-TNF alpha autoantibodies in plasma and from EBV transformed lymphocytes of autoimmune and normal individuals.

To examine the ability of normal and autoimmune individuals to produce circulating anti-TNF alpha antibodies, plasma samples from 10 RA patients, 10 SLE patients and 5 normal subjects were assessed for anti-TNF alpha antibody. While every individual tested demonstrated circulating IgM anti-TNF alpha antibody, IgG anti-TNF alpha autoantibody was seen predominantly in autoimmune patients. Only 1 of 5 normal individuals, but 15 of 20 autoimmune individuals had plasma IgG anti-TNF alpha antibodies. To examine the ability of normal and autoimmune individuals to produce anti-TNF alpha autoantibody from their circulating lymphocytes, EBV transformation was performed. Oligoclonal immortal cell lines were successfully established from 13 patients and each one secreted detectable IgM anti-TNF alpha autoantibody. Transformed cells from only 1 of 5 normal individuals secreted IgM anti-TNF alpha autoantibody. These results indicate a higher prevalence of anti-TNF alpha autoantibody production among autoimmune individuals although normal individuals are also capable of producing these autoantibodies.

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