大鼠e -选择素补体调节结构域mRNA剪接变异的证据。

K L Billups, J L Sherley, M A Palladino, J W Tindall, K P Roberts
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引用次数: 0

摘要

粘附蛋白e -选择素是急性炎症期间内皮细胞-白细胞相互作用的一种介质。为了研究e -选择素功能的分子调控,我们检测了靶大鼠组织中e -选择素mRNA在脂多糖(一种有效的急性炎症诱导剂)作用后的表达。在这些研究过程中,我们分离了两个独特的大鼠e -选择素cDNA片段。这两个cDNA片段与先前分离的小鼠和人类e -选择素cDNA具有广泛的核苷酸序列同源性。然而,它们的不同之处在于编码补体调控结构域5 (CR5)的序列。先前的研究表明,不同动物物种表达的e -选择素mrna编码不同数量的CR结构域。这两个大鼠e -选择素cDNA片段的分离,仅在CR5的存在上有所不同,代表了在同一物种中存在e -选择素cr变异mrna的第一个直接证据。此外,CR5(-) cDNA的序列与其来自CR5(+) mRNA的mRNA剪接变体的起源一致。我们已经证明在大鼠心脏组织中存在两种预测的mRNA物种,并研究了它们在脂多糖反应中的表达。尽管这两种mRNA变体都是由脂多糖诱导的,但CR5(-)形式在处理组织和对照组织中都更丰富。mRNA丰度的差异可能表明不同水平的CR5变异蛋白在e -选择素依赖的炎症过程中执行不同的功能任务。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Evidence for E-selectin complement regulatory domain mRNA splice variants in the rat.

The adhesion protein E-selectin is one mediator of endothelial cell-leukocyte interaction during acute inflammation. To investigate the molecular regulation of E-selectin function, we have examined the expression of E-selectin mRNA in target rat tissues after administration of lipopolysaccharide, a potent inducer of acute inflammation. In the course of these studies we isolated two unique rat E-selectin cDNA fragments. Both cDNA fragments show extensive nucleotide sequence homology to previously isolated mouse and human E-selectin cDNAs. However, they differ for the presence of sequences that encode complement regulatory domain-5 (CR5). Previous studies have shown that different animal species express E-selectin mRNAs that encode different numbers of CR domains. The isolation of these two rat E-selectin cDNA fragments, which differ only for the presence of CR5, represents the first direct evidence for the existence of E-selectin CR-variant mRNAs in the same species. Moreover, the sequence of the CR5(-) cDNA is consistent with its origin from an mRNA splice variant of a CR5(+) mRNA. We have demonstrated the presence of the two predicted mRNA species in rat heart tissue and have investigated their expression in response to lipopolysaccharide. Although both mRNA variants were greatly induced by lipopolysaccharide, the CR5(-) form was more abundant in both treated and control tissues. This difference in mRNA abundance may indicate different levels of CR5 variant proteins that perform functionally distinct tasks in E-selectin dependent inflammatory processes.

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