蛋白质表面张力的测定2。血清白蛋白的表面张力,在蛋白质-空气界面处改变

D.R. Absolom , C.J. Van Oss , W. Zingg , A.W. Neumann
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引用次数: 56

摘要

血清白蛋白本身的表面张力为⋍70.3 erg/cm2,当溶解在水中时,水的表面张力从72.5降低到⋍50 erg/cm2,这是通过多种方法测量的,包括垂坠滴法、威廉平板法和铂环法。用接触角法,在一层薄薄的白蛋白上发现了同样低甚至更低的表面张力,这层白蛋白被吸附在低能表面上,随后暴露在空气中。在接触角法下,浓度在0.01 ~ 5.5% (w/v)之间的白蛋白溶液滴的表面张力仅在67 ~ 61 erg/cm2之间变化。通过垂坠滴法、威廉板法和铂环法,人们基本上可以测量气液界面的表面张力,蛋白质倾向于在此吸附,并且可以预期可逆或不可逆的重新定向。同样的道理也适用于吸附在低能量表面并暴露在空气中的薄薄的一层蛋白质。因此,当通过表面张力测量的行为,或在将蛋白质吸附到底物上后,蛋白质暴露在气液界面时,它显然失去了其天然水合状态的明显亲水性特征,并通过重定向表现出更疏水的三级构型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of surface tensions of proteins II. Surface tension of serum albumin, altered at the protein-air interface

Serum albumin, which itself has a surface tension of ⋍70.3 erg/cm2, when dissolved in water lowers the surface tension of water from 72.5 to ⋍50 erg/cm2, as measured by a variety of means, including the pendant drop, the Wilhelmy plate and the platinum ring methods. Equally low and even lower surface tensions are found with the contact angle method, on a thin layer of albumin that had been adsorbed onto a low energy surface and subsequently exposed to air. Surface tensions of drops of albumin solutions varying in concentration from 0.01 to 5.5% (w/v) yielded, with a contact angle method, values that only varied between 67 and 61 erg/cm2. With the pendant drop, the Wilhelmy plate and the platinum ring methods, one essentially measures the surface tension at the air-liquid interface, at which proteins tend to adsorb, and where reversible or irreversible reorientation can be expected. The same holds for a thin layer of protein adsorbed onto a low energy surface, exposed to air. Thus, when through the very act of surface tension measurement, or after adsorbing protein onto a substrate, protein is exposed at the air-liquid interface, it apparently loses the pronounced hydrophilicity characteristic of its native hydrated state and manifests through reorientation a much more hydrophobic tertiary configuration.

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