用染料激光闪光光解法研究了人红细胞悬浮液中一氧化碳和氧气与t态血红蛋白的反应动力学。

Physiological chemistry and physics Pub Date : 1982-01-01
B B Hasinoff
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引用次数: 0

摘要

用脉冲宽度为300 ns的染料激光光解HbCO或HbO2,研究了人红细胞(RBC)悬浮液中一氧化碳和氧与慢反应t态脱氧血红蛋白(HbT)的反应动力学。速率常数仅使用缓慢HbT反应的前10%来确定,因为在较长的时间内,配体从RBC扩散严重影响动力学。测定的速率常数与溶液中的速率常数相似,表明红细胞中不同的物理环境对血红蛋白动力学的影响不大。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Kinetics of the reaction of carbon monoxide and oxygen with T-state hemoglobin in human red blood cell suspensions studied by dye laser flash photolysis.

The reaction kinetics of carbon monoxide and oxygen binding to slow reacting T-state "deoxy" hemoglobin (HbT) in human red blood cell (RBC) suspensions were studied with a 300-ns pulse width dye laser to photodissociate either HbCO or HbO2. The rate constants were determined using only the first 10% of the slow HbT reaction, as at longer times diffusion of ligand away from the RBC seriously affects the kinetics. The rate constants measured are similar to those in solution, indicating that the different physical environment in the RBC has little effect on the Hb kinetics.

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