{"title":"分枝杆菌和分枝噬菌体DNA中的核酸碱基甲基化。","authors":"P A Somogyi, M Maso Bel, I Földes","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Methylated bases of the DNA of two mycobacteria (Mycobacterium phlei and Mycobacterium smegmatis var. butyricum) and two mycobacteriophages (Phage phlei and Phage butyricum) have been studied. In both the bacterial and the phage DNAs 5-methyl-cytosine and 6-methyl-aminopurine could be detected. Using L-(methyl-H3)-methionine as methyl donor not only the methylated bases of bacterium and phage DNA proved to be radioactive, but also the non-methylated purine residues and thymine. Possible pathways of this phenomenon are discussed.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"29 3","pages":"181-5"},"PeriodicalIF":0.0000,"publicationDate":"1982-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Methylated nucleic acid bases in Mycobacterium and mycobacteriophage DNA.\",\"authors\":\"P A Somogyi, M Maso Bel, I Földes\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Methylated bases of the DNA of two mycobacteria (Mycobacterium phlei and Mycobacterium smegmatis var. butyricum) and two mycobacteriophages (Phage phlei and Phage butyricum) have been studied. In both the bacterial and the phage DNAs 5-methyl-cytosine and 6-methyl-aminopurine could be detected. Using L-(methyl-H3)-methionine as methyl donor not only the methylated bases of bacterium and phage DNA proved to be radioactive, but also the non-methylated purine residues and thymine. Possible pathways of this phenomenon are discussed.</p>\",\"PeriodicalId\":75387,\"journal\":{\"name\":\"Acta microbiologica Academiae Scientiarum Hungaricae\",\"volume\":\"29 3\",\"pages\":\"181-5\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1982-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta microbiologica Academiae Scientiarum Hungaricae\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Academiae Scientiarum Hungaricae","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Methylated nucleic acid bases in Mycobacterium and mycobacteriophage DNA.
Methylated bases of the DNA of two mycobacteria (Mycobacterium phlei and Mycobacterium smegmatis var. butyricum) and two mycobacteriophages (Phage phlei and Phage butyricum) have been studied. In both the bacterial and the phage DNAs 5-methyl-cytosine and 6-methyl-aminopurine could be detected. Using L-(methyl-H3)-methionine as methyl donor not only the methylated bases of bacterium and phage DNA proved to be radioactive, but also the non-methylated purine residues and thymine. Possible pathways of this phenomenon are discussed.
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