[人给药后血浆醋酸酯的气相色谱测定方法[作者译]。

R Bruno, Y Santoni, A Botta, J P Cano
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引用次数: 0

摘要

在存在内标的情况下,用乙醇在碱性培养基中提取血浆醋酸盐。水醇相浓缩后,在w上用10%的sp1200和1%的H3PO4进行色谱分析。在0.05 ~ 10 mM/I范围内测试了该方法的线性,在0.1 ~ 10 mM/I范围内的重现性为+/- 3.5%,小于0.1 mM/I的重现性为+/- 10%。该方法具有足够的灵敏度,可以评估生理上的乙酸水平和乙醇生物转化后的乙酸水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
[Method of plasma acetate determination by gas chromatography after ethanol administration in man (author's transl)].

Plasma acetate is extracted using ethanol in alkaline medium, in presence of internal standard. After concentration of the hydroalcoholic phase, the chromatographic analysis is performed utilizing 10% SP 1200 and 1% H3PO4 on chromosorb W. The linearity of the method has been tested from 0.05 to 10 mM/I, its reproducibility is +/- 3.5% for levels ranging from 0.1 to 10 mM/I and +/- 10% for those smaller than 0.1 mM/I. This method is sensitive enough to assess the physiological acetate levels and those obtained after ethanol biotransformation.

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